InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: SUPT4H1

Summary

InnateDB Protein

IDBP-61126.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

SUPT4H1

Protein Name

suppressor of Ty 4 homolog 1 (S. cerevisiae)

Synonyms

SPT4; SPT4H; SUPT4H;

Species

Homo sapiens

Ensembl Protein

ENSP00000225504

InnateDB Gene

IDBG-244315 (SUPT4H1)

Protein Structure

UniProt Annotation

Function

Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV- 1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. {ECO:0000269PubMed:10075709, ECO:0000269PubMed:10199401, ECO:0000269PubMed:10454543, ECO:0000269PubMed:10912001, ECO:0000269PubMed:11112772, ECO:0000269PubMed:11553615, ECO:0000269PubMed:12653964, ECO:0000269PubMed:12718890, ECO:0000269PubMed:15136722, ECO:0000269PubMed:15380072, ECO:0000269PubMed:16214896, ECO:0000269PubMed:9450929, ECO:0000269PubMed:9857195}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:8649394}.

Disease Associations

Tissue Specificity

Widely expressed. {ECO:0000269PubMed:8649394, ECO:0000269PubMed:8786137}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 16 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	16 [view]
Protein-Protein	15 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0046982	protein heterodimerization activity

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006338	chromatin remodeling
GO:0006355	regulation of transcription, DNA-templated
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0006366	transcription from RNA polymerase II promoter
GO:0006368	transcription elongation from RNA polymerase II promoter
GO:0010467	gene expression
GO:0016032	viral process
GO:0032785	negative regulation of DNA-dependent transcription, elongation
GO:0032786	positive regulation of DNA-templated transcription, elongation
GO:0034244	negative regulation of transcription elongation from RNA polymerase II promoter
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0050434	positive regulation of viral transcription