InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Dnmt3a

Summary

InnateDB Protein

IDBP-622427.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Dnmt3a

Protein Name

DNA methyltransferase 3A

Synonyms

MmuIIIA;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000134009

InnateDB Gene

IDBG-127800 (Dnmt3a)

Protein Structure

UniProt Annotation

Function

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. {ECO:0000269PubMed:10555141, ECO:0000269PubMed:11350943, ECO:0000269PubMed:11399089, ECO:0000269PubMed:11919202, ECO:0000269PubMed:15215868, ECO:0000269PubMed:16567415, ECO:0000269PubMed:17713477, ECO:0000269PubMed:18823905}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:12138111, ECO:0000269PubMed:15456878, ECO:0000269PubMed:20547484}. Cytoplasm {ECO:0000250}. Note=Accumulates in the major satellite repeats at pericentric heterochromatin.

Disease Associations

Tissue Specificity

Isoform 1 is expressed ubiquitously at low levels. Expression of isoform 2 is restricted to tissues containing cells which are undergoing active de novo methylation, including spleen, testis and thymus. {ECO:0000269PubMed:12138111}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 28 experimentally validated interaction(s) in this database.
They are also associated with 39 interaction(s) predicted by orthology.

Experimentally validated
Total	28 [view]
Protein-Protein	27 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	39 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003682	chromatin binding
GO:0003886	DNA (cytosine-5-)-methyltransferase activity
GO:0005515	protein binding
GO:0008168	methyltransferase activity
GO:0045322	unmethylated CpG binding
GO:0046872	metal ion binding
GO:0051718	DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006306	DNA methylation
GO:0006346	methylation-dependent chromatin silencing
GO:0006349	regulation of gene expression by genetic imprinting
GO:0006351	transcription, DNA-templated
GO:0007283	spermatogenesis
GO:0010216	maintenance of DNA methylation
GO:0010424	DNA methylation on cytosine within a CG sequence
GO:0043045	DNA methylation involved in embryo development
GO:0043046	DNA methylation involved in gamete generation
GO:0044027	hypermethylation of CpG island
GO:0046498	S-adenosylhomocysteine metabolic process
GO:0046499	S-adenosylmethioninamine metabolic process
GO:0071230	cellular response to amino acid stimulus
GO:0090116	C-5 methylation of cytosine

Cellular Component

GO:0000775	chromosome, centromeric region
GO:0000791	euchromatin
GO:0000792	heterochromatin
GO:0005634	nucleus
GO:0005720	nuclear heterochromatin
GO:0005737	cytoplasm
GO:0016363	nuclear matrix

Protein Structure and Domains

PDB ID

MGI:1261827

InterPro

IPR000313 PWWP domain
IPR001525 C-5 cytosine methyltransferase
IPR011011 Zinc finger, FYVE/PHD-type
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase-like

PFAM

PF00855
PF00145

PRINTS

PR00105

PIRSF

SMART

SM00293

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt