InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Clp1

Summary

InnateDB Protein

IDBP-623979.2

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Clp1

Protein Name

CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae)

Synonyms

AI462438; Heab;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000129300

InnateDB Gene

IDBG-185205 (Clp1)

Protein Structure

UniProt Annotation

Function

Polynucleotide kinase that can phosphorylate the 5'- hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:23474986, PubMed:24766809). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'- terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing. {ECO:0000269PubMed:23474986, ECO:0000269PubMed:24766809}.

Subcellular Localization

Nucleus {ECO:0000255HAMAP-Rule:MF_03035}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 10 experimentally validated interaction(s) in this database.
They are also associated with 13 interaction(s) predicted by orthology.

Experimentally validated
Total	10 [view]
Protein-Protein	10 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	13 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005524	ATP binding
GO:0046404	ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity
GO:0051733	polydeoxyribonucleotide kinase activity
GO:0051736	ATP-dependent polyribonucleotide 5'-hydroxyl-kinase activity

Biological Process

GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation
GO:0008219	cell death
GO:0016310	phosphorylation
GO:0021695	cerebellar cortex development
GO:0030423	targeting of mRNA for destruction involved in RNA interference
GO:0031124	mRNA 3'-end processing
GO:0035087	siRNA loading onto RISC involved in RNA interference

Cellular Component

GO:0000214	tRNA-intron endonuclease complex
GO:0005849	mRNA cleavage factor complex

Protein Structure and Domains

PDB ID

MGI:2138968

InterPro

IPR010655 Pre-mRNA cleavage complex II Clp1
IPR027417 P-loop containing nucleoside triphosphate hydrolase
IPR029007 MobB-type P-loop domain

PFAM

PF06807
PF03205

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q99LI9

PhosphoSite

PhosphoSite-Q99LI9

TrEMBL

UniProt Splice Variant

Entrez Gene

98985

UniGene

Mm.403232

RefSeq

MGI ID

MGI Symbol

Clp1

OMIM

CCDS

CCDS16191

HPRD

IMGT

EMBL

AK077647 AK145209 AK169709 BC003237

GenPept

AAH03237 BAC36924 BAE26299 BAE41321