InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Nisch

Summary

InnateDB Protein

IDBP-624607.2

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Nisch

Protein Name

nischarin

Synonyms

1200007D05Rik; 3202002H23Rik; AW494485; I-1;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000132842

InnateDB Gene

IDBG-145371 (Nisch)

Protein Structure

UniProt Annotation

Function

Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1- stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis (By similarity). Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3 (By similarity). When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures (By similarity). {ECO:0000250}.

Subcellular Localization

Cell membrane {ECO:0000250}. Cytoplasm {ECO:0000269PubMed:11121431, ECO:0000269PubMed:15229651, ECO:0000269PubMed:16002401, ECO:0000269PubMed:16678176}. Early endosome {ECO:0000250}. Recycling endosome {ECO:0000250}. Note=Enriched in the early/sorting and recycling endosomes (By similarity). Colocalized in early/sorting endosomes with EEA1 and SNX2 and in recycling endosomes with transferrin receptor (By similarity). Colocalized with MAPK1 and MAPK3 in RVLM neurons (By similarity). Detected in the perinuclear region partially associated with punctate structures. Colocalizes with PAK1 in cytoplasm, vesicular structures in the perinuclear area and membrane ruffles. Colocalizes with RAC1 in the cytoplasm and vesicles structures. {ECO:0000250}.

Disease Associations

Tissue Specificity

Highly expressed in brain and kidney. Moderately expressed in heart, liver, lung and skeletal muscle. Not detected in spleen and testis. {ECO:0000269PubMed:11121431}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005178	integrin binding
GO:0005515	protein binding
GO:0008227	G-protein coupled amine receptor activity
GO:0035091	phosphatidylinositol binding

Biological Process

GO:0006006	glucose metabolic process
GO:0006915	apoptotic process
GO:0007186	G-protein coupled receptor signaling pathway
GO:0008217	regulation of blood pressure
GO:0016601	Rac protein signal transduction
GO:0030036	actin cytoskeleton organization
GO:0030336	negative regulation of cell migration
GO:0032228	regulation of synaptic transmission, GABAergic
GO:0048243	norepinephrine secretion