InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Slit2

Summary

InnateDB Protein

IDBP-627903.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Slit2

Protein Name

slit homolog 2 (Drosophila)

Synonyms

Drad-1; E030015M03Rik; E130320P19Rik; mKIAA4141; Slil3; slit-2;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000127615

InnateDB Gene

IDBG-162862 (Slit2)

Protein Structure

UniProt Annotation

Function

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system, appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration (By similarity). {ECO:0000250}.

Subcellular Localization

Secreted {ECO:0000250}. Note=The C-terminal cleavage protein is more diffusible than the larger N-terminal protein that is more tightly cell associated. {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in developing eye, in the optic stalk, and in the ventral diencephalon. {ECO:0000269PubMed:10864954}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005095	GTPase inhibitor activity
GO:0005102	receptor binding
GO:0005509	calcium ion binding
GO:0005515	protein binding
GO:0008201	heparin binding
GO:0042802	identical protein binding
GO:0042803	protein homodimerization activity
GO:0043237	laminin-1 binding
GO:0043394	proteoglycan binding
GO:0045499	chemorepellent activity
GO:0048495	Roundabout binding

Biological Process

GO:0001656	metanephros development
GO:0001657	ureteric bud development
GO:0001701	in utero embryonic development
GO:0001933	negative regulation of protein phosphorylation
GO:0002042	cell migration involved in sprouting angiogenesis
GO:0002689	negative regulation of leukocyte chemotaxis
GO:0007409	axonogenesis
GO:0007411	axon guidance
GO:0008045	motor neuron axon guidance
GO:0008285	negative regulation of cell proliferation
GO:0010593	negative regulation of lamellipodium assembly
GO:0010596	negative regulation of endothelial cell migration
GO:0010629	negative regulation of gene expression
GO:0014912	negative regulation of smooth muscle cell migration
GO:0016337	single organismal cell-cell adhesion
GO:0021772	olfactory bulb development
GO:0021836	chemorepulsion involved in postnatal olfactory bulb interneuron migration
GO:0021972	corticospinal neuron axon guidance through spinal cord
GO:0022029	telencephalon cell migration
GO:0030308	negative regulation of cell growth
GO:0030336	negative regulation of cell migration
GO:0030517	negative regulation of axon extension
GO:0030837	negative regulation of actin filament polymerization
GO:0031290	retinal ganglion cell axon guidance
GO:0032870	cellular response to hormone stimulus
GO:0033563	dorsal/ventral axon guidance
GO:0043065	positive regulation of apoptotic process
GO:0043086	negative regulation of catalytic activity
GO:0043116	negative regulation of vascular permeability
GO:0048754	branching morphogenesis of an epithelial tube
GO:0048812	neuron projection morphogenesis
GO:0048846	axon extension involved in axon guidance
GO:0050919	negative chemotaxis
GO:0050929	induction of negative chemotaxis
GO:0051058	negative regulation of small GTPase mediated signal transduction
GO:0051414	response to cortisol
GO:0060603	mammary gland duct morphogenesis
GO:0060763	mammary duct terminal end bud growth
GO:0061364	apoptotic process involved in luteolysis
GO:0070100	negative regulation of chemokine-mediated signaling pathway
GO:0071504	cellular response to heparin
GO:0071672	negative regulation of smooth muscle cell chemotaxis
GO:0071676	negative regulation of mononuclear cell migration
GO:0090024	negative regulation of neutrophil chemotaxis
GO:0090260	negative regulation of retinal ganglion cell axon guidance
GO:0090288	negative regulation of cellular response to growth factor stimulus

Cellular Component

GO:0005615	extracellular space
GO:0005737	cytoplasm
GO:0009986	cell surface
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

MGI:1315205

InterPro

IPR000372 Leucine-rich repeat-containing N-terminal
IPR000483 Cysteine-rich flanking region, C-terminal
IPR000742 Epidermal growth factor-like domain
IPR001611 Leucine-rich repeat
IPR001791 Laminin G domain
IPR001881 EGF-like calcium-binding domain
IPR003591 Leucine-rich repeat, typical subtype
IPR003645 Follistatin-like, N-terminal
IPR006207 Cystine knot, C-terminal
IPR008985 Concanavalin A-like lectin/glucanases superfamily

PFAM