InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: DDX5

Summary

InnateDB Protein

IDBP-64776.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

DDX5

Protein Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 5

Synonyms

G17P1; HLR1; HUMP68; p68;

Species

Homo sapiens

Ensembl Protein

ENSP00000225792

InnateDB Gene

IDBG-64774 (DDX5)

Protein Structure

UniProt Annotation

Function

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. {ECO:0000269PubMed:10409727, ECO:0000269PubMed:11250900, ECO:0000269PubMed:12527917, ECO:0000269PubMed:15298701, ECO:0000269PubMed:15660129, ECO:0000269PubMed:17011493, ECO:0000269PubMed:17960593, ECO:0000269PubMed:18829551, ECO:0000269PubMed:19718048, ECO:0000269PubMed:21343338}.

Subcellular Localization

Nucleus, nucleolus {ECO:0000269PubMed:10837141, ECO:0000269PubMed:1996094}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 230 experimentally validated interaction(s) in this database.
They are also associated with 11 interaction(s) predicted by orthology.

Experimentally validated
Total	230 [view]
Protein-Protein	221 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	6 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	11 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003712	transcription cofactor activity
GO:0003713	transcription coactivator activity
GO:0003724	RNA helicase activity
GO:0004004	ATP-dependent RNA helicase activity
GO:0005515	protein binding
GO:0005516	calmodulin binding
GO:0005524	ATP binding
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
GO:0019899	enzyme binding
GO:0030331	estrogen receptor binding
GO:0036002	pre-mRNA binding
GO:0044822	poly(A) RNA binding
GO:0048306	calcium-dependent protein binding
GO:0050681	androgen receptor binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0000381	regulation of alternative mRNA splicing, via spliceosome
GO:0000398	mRNA splicing, via spliceosome
GO:0001701	in utero embryonic development
GO:0006200	ATP catabolic process
GO:0006351	transcription, DNA-templated
GO:0007623	circadian rhythm
GO:0016049	cell growth
GO:0033148	positive regulation of intracellular estrogen receptor signaling pathway
GO:0043517	positive regulation of DNA damage response, signal transduction by p53 class mediator
GO:0045069	regulation of viral genome replication
GO:0045667	regulation of osteoblast differentiation
GO:0045893	positive regulation of transcription, DNA-templated
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0060765	regulation of androgen receptor signaling pathway
GO:0072332	intrinsic apoptotic signaling pathway by p53 class mediator
GO:2001014	regulation of skeletal muscle cell differentiation

Cellular Component

GO:0005634	nucleus
GO:0005730	nucleolus
GO:0016020	membrane
GO:0030529	ribonucleoprotein complex
GO:0070062	extracellular vesicular exosome
GO:0071013	catalytic step 2 spliceosome

Protein Structure and Domains

PDB ID

InterPro

IPR001650 Helicase, C-terminal
IPR011545 DEAD/DEAH box helicase domain
IPR012587 P68HR
IPR014001 Helicase, superfamily 1/2, ATP-binding domain
IPR014014 RNA helicase, DEAD-box type, Q motif
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00271
PF00270
PF08061

PRINTS

PIRSF

SMART

SM00490
SM00487

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P17844