InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: WIPI1

Summary

InnateDB Protein

IDBP-65868.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

WIPI1

Protein Name

WD repeat domain, phosphoinositide interacting 1

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000262139

InnateDB Gene

IDBG-65866 (WIPI1)

Protein Structure

UniProt Annotation

Function

Plays an important role in autophagy and in particular starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions upstream of the ATG12-ATG5-ATG16L1 complex and LC3, and downstream of the ULK1 and PI3-kinase complexes. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation- induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway. {ECO:0000269PubMed:15020712, ECO:0000269PubMed:15602573, ECO:0000269PubMed:20114074, ECO:0000269PubMed:20484055, ECO:0000269PubMed:20639694, ECO:0000269PubMed:21317285, ECO:0000269PubMed:22829830, ECO:0000269PubMed:23088497}.

Subcellular Localization

Golgi apparatus, trans-Golgi network. Endosome. Cytoplasmic vesicle, clathrin-coated vesicle. Preautophagosomal structure membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton. Note=Trans elements of the Golgi and peripheral endosomes. Dynamically cycles through these compartments and is susceptible to conditions that modulate membrane flux. Enriched in clathrin-coated vesicles. Upon starvation-induced autophagy, accumulates at subcellular structures in the cytoplasm: enlarged vesicular and lasso-like structures, and large cup-shaped structures predominantly around the nucleus. Recruitment to autophagic membranes is controlled by MTMR14. Labile microtubules specifically recruit markers of autophagosome formation like WIPI1, whereas mature autophagosomes may bind to stable microtubules.

Disease Associations

Tissue Specificity

Ubiquitously expressed. Highly expressed in skeletal muscle, heart, testis, pancreas and placenta. Highly expressed in G361, Sk-mel-28, Sk-mel-13, WM852 and WM451 cells. Up-regulated in a variety of tumor tissues. {ECO:0000269PubMed:15602573}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 8 experimentally validated interaction(s) in this database.

Experimentally validated
Total	8 [view]
Protein-Protein	8 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005102	receptor binding
GO:0005515	protein binding
GO:0030331	estrogen receptor binding
GO:0032266	phosphatidylinositol-3-phosphate binding
GO:0050681	androgen receptor binding
GO:0080025	phosphatidylinositol-3,5-bisphosphate binding

Biological Process

GO:0006914	autophagy
GO:0006987	activation of signaling protein activity involved in unfolded protein response
GO:0030968	endoplasmic reticulum unfolded protein response
GO:0044267	cellular protein metabolic process
GO:0048203	vesicle targeting, trans-Golgi to endosome

Cellular Component

GO:0000139	Golgi membrane
GO:0000407	pre-autophagosomal structure
GO:0000421	autophagic vacuole membrane
GO:0005737	cytoplasm
GO:0005802	trans-Golgi network
GO:0005856	cytoskeleton
GO:0010008	endosome membrane
GO:0030136	clathrin-coated vesicle
GO:0034045	pre-autophagosomal structure membrane