Version 5.4
Bos taurus Protein: EPB49
Summary
InnateDB Protein IDBP-686332.2
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol EPB49
Protein Name Dematin
Synonyms EPB49;
Species Bos taurus
Ensembl Protein ENSBTAP00000054225
InnateDB Gene IDBG-634536 (EPB49)
Protein Structure
UniProt Annotation
Function Membrane-cytoskeleton-associated protein with F-actin- binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Plays also a role as a modulator of actin dynamics in fibroblasts; acts as negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation (By similarity). {ECO:0000250}.
Subcellular Localization Cytoplasm {ECO:0000250}. Cytoplasm, cytosol {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Cell membrane {ECO:0000250}. Membrane {ECO:0000250}. Endomembrane system {ECO:0000250}. Cell projection {ECO:0000250}. Note=Localized at the spectrin-actin junction of erythrocyte plasma membrane. Localized to intracellular membranes and the cytoskeletal network. Localized at intracellular membrane-bounded organelle compartment in platelets that likely represent the dense tubular network membrane (By similarity). {ECO:0000250}.
Disease Associations
Tissue Specificity
Comments
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 8 interaction(s) predicted by orthology.
Predicted by orthology
Total 8 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003779 actin binding
GO:0005102 receptor binding
GO:0005515 protein binding
GO:0030507 spectrin binding
GO:0043621 protein self-association
Biological Process
GO:0006461 protein complex assembly
GO:0007010 cytoskeleton organization
GO:0008360 regulation of cell shape
GO:0010591 regulation of lamellipodium assembly
GO:0010763 positive regulation of fibroblast migration
GO:0010801 negative regulation of peptidyl-threonine phosphorylation
GO:0010812 negative regulation of cell-substrate adhesion
GO:0030036 actin cytoskeleton organization
GO:0030194 positive regulation of blood coagulation
GO:0032956 regulation of actin cytoskeleton organization
GO:0033137 negative regulation of peptidyl-serine phosphorylation
GO:0035584 calcium-mediated signaling using intracellular calcium source
GO:0035585 calcium-mediated signaling using extracellular calcium source
GO:0048821 erythrocyte development
GO:0050732 negative regulation of peptidyl-tyrosine phosphorylation
GO:0051017 actin filament bundle assembly
GO:0051489 regulation of filopodium assembly
GO:0051693 actin filament capping
GO:0051895 negative regulation of focal adhesion assembly
GO:0070560 protein secretion by platelet
GO:0071277 cellular response to calcium ion
GO:0071320 cellular response to cAMP
GO:0090303 positive regulation of wound healing
GO:0090315 negative regulation of protein targeting to membrane
GO:0090527 actin filament reorganization
GO:1900025 negative regulation of substrate adhesion-dependent cell spreading
GO:1900026 positive regulation of substrate adhesion-dependent cell spreading
GO:1901731 positive regulation of platelet aggregation
GO:2001046 positive regulation of integrin-mediated signaling pathway
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005884 actin filament
GO:0005886 plasma membrane
GO:0012505 endomembrane system
GO:0014731 spectrin-associated cytoskeleton
GO:0016020 membrane
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0030863 cortical cytoskeleton
GO:0031095 platelet dense tubular network membrane
GO:0031253 cell projection membrane
GO:0042995 cell projection
GO:0048471 perinuclear region of cytoplasm
Protein Structure and Domains
PDB ID
InterPro IPR003128 Villin headpiece
PFAM PF02209
PRINTS
PIRSF
SMART SM00153
TIGRFAMs
Post-translational Modifications
Modification
Cross-References
SwissProt Q08DM1
PhosphoSite PhosphoSite-
TrEMBL
UniProt Splice Variant
Entrez Gene 512981
UniGene Bt.33159
RefSeq
HUGO
OMIM
CCDS
HPRD
IMGT
EMBL BC123672
GenPept AAI23673

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

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Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca