InnateDB: Systems Biology of the Innate Immune Response

Bos taurus Protein: RPE65

Summary

InnateDB Protein

IDBP-690121.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RPE65

Protein Name

retinoid isomerohydrolase

Synonyms

Species

Bos taurus

Ensembl Protein

ENSBTAP00000041254

InnateDB Gene

IDBG-638059 (RPE65)

Protein Structure

UniProt Annotation

Function

Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis- retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association. {ECO:0000269PubMed:16096063, ECO:0000269PubMed:19805034, ECO:0000269PubMed:20100834}.

Subcellular Localization

Cytoplasm. Cell membrane; Lipid-anchor. Microsome membrane. Note=Undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells (By similarity). Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated. {ECO:0000250}.

Disease Associations

Tissue Specificity

Retinal pigment epithelium specific. {ECO:0000269PubMed:19805034, ECO:0000269PubMed:20100834}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004744	retinal isomerase activity
GO:0016787	hydrolase activity
GO:0016853	isomerase activity
GO:0046872	metal ion binding
GO:0052884	all-trans-retinyl-palmitate hydrolase, 11-cis retinol forming activity
GO:0052885	all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity

Biological Process

GO:0001895	retina homeostasis
GO:0007468	regulation of rhodopsin gene expression
GO:0007601	visual perception
GO:0008286	insulin receptor signaling pathway
GO:0042572	retinol metabolic process
GO:0042574	retinal metabolic process
GO:0050896	response to stimulus
GO:0050908	detection of light stimulus involved in visual perception
GO:0060041	retina development in camera-type eye
GO:0060042	retina morphogenesis in camera-type eye
GO:0071257	cellular response to electrical stimulus

Cellular Component

GO:0005737	cytoplasm
GO:0005783	endoplasmic reticulum
GO:0005886	plasma membrane
GO:0016020	membrane
GO:0031090	organelle membrane
GO:0043231	intracellular membrane-bounded organelle

Protein Structure and Domains

PDB ID

InterPro

IPR004294 Carotenoid oxygenase

PFAM

PF03055

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q28175

PhosphoSite

PhosphoSite-

TrEMBL

UniProt Splice Variant

Entrez Gene

282043

UniGene

Bt.108

RefSeq

NP_776878

HUGO

OMIM

CCDS

HPRD

IMGT

EMBL

L11356 X66277

GenPept

AAC37306 CAA46988