InnateDB: Systems Biology of the Innate Immune Response

Bos taurus Protein: ALYREF

Summary

InnateDB Protein

IDBP-694803.2

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

ALYREF

Protein Name

THO complex subunit 4

Synonyms

THOC4;

Species

Bos taurus

Ensembl Protein

ENSBTAP00000011202

InnateDB Gene

IDBG-642357 (ALYREF)

Protein Structure

UniProt Annotation

Function

Export adapter involved in nuclear export of spliced and unspliced mRNA. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NFX1 pathway). Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm. TREX recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. Required for TREX complex assembly and for linking DDX39B to the cap-binding complex (CBC). In conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in the nuclear export of intronless mRNA; proposed to be recruited to intronless mRNA by ATP-bound DDX39B. Involved in transcription elongation and genome stability (By similarity). {ECO:0000250}.Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation. {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000250}. Nucleus speckle {ECO:0000250}. Cytoplasm {ECO:0000250}. Note=Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 0 experimentally validated interaction(s) in this database.
They are also associated with 68 interaction(s) predicted by orthology.

Predicted by orthology
Total	68 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003723	RNA binding
GO:0005515	protein binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0000018	regulation of DNA recombination
GO:0001649	osteoblast differentiation
GO:0006397	mRNA processing
GO:0006406	mRNA export from nucleus
GO:0006810	transport
GO:0008380	RNA splicing
GO:0031297	replication fork processing
GO:0032786	positive regulation of DNA-templated transcription, elongation
GO:0046784	intronless viral mRNA export from host nucleus
GO:0051028	mRNA transport

Cellular Component

GO:0000346	transcription export complex
GO:0005634	nucleus
GO:0005681	spliceosomal complex
GO:0005737	cytoplasm
GO:0016020	membrane
GO:0016607	nuclear speck
GO:0035145	exon-exon junction complex
GO:0070062	extracellular vesicular exosome
GO:0071013	catalytic step 2 spliceosome

Protein Structure and Domains

PDB ID

InterPro

IPR000504 RNA recognition motif domain

PFAM

PF00076

PRINTS

PIRSF

SMART

SM00360

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q3T0I4

PhosphoSite

PhosphoSite-

TrEMBL

UniProt Splice Variant

Entrez Gene

537706

UniGene

Bt.48992

RefSeq

NP_001030494

HUGO

OMIM

CCDS

HPRD

IMGT

EMBL

BC102384

GenPept

AAI02385