InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: WNK3

Summary

InnateDB Protein

IDBP-71043.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

WNK3

Protein Name

WNK lysine deficient protein kinase 3

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000364301

InnateDB Gene

IDBG-71035 (WNK3)

Protein Structure

UniProt Annotation

Function

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Phosphorylates WNK4. Regulates the phosphorylation of SLC12A1 and SLC12A2. Increases Ca(2+) influx mediated by TRPV5 and TRPV6 by enhancing their membrane expression level via a kinase-dependent pathway. Inhibits the activity of KCNJ1 by decreasing its expression at the cell membrane in a non-catalytic manner.Isoform 1, isoform 2, isoform 3 and isoform 4 stimulate the activity of SLC12A1, SLC12A2 and SLC12A3 and inhibit the activity of SLC12A4, SLC12A5, SLC12A6 and SLC12A7. According to PubMed:19470686, isoform 1 inhibits the activity of SLC12A3.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:16501604}.

Disease Associations

Tissue Specificity

Expressed in brain, lung, kidney, liver and pancreas, and in fetal tissues including placenta, fetal brain, lung and kidney. Very low levels of expression were also detected in fetal heart, thymus, liver and spleen. Isoform 1 is brain- specific. Isoform 3 is kidney-specific. {ECO:0000269PubMed:11571656, ECO:0000269PubMed:15194194, ECO:0000269PubMed:16275913}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 10 experimentally validated interaction(s) in this database.

Experimentally validated
Total	10 [view]
Protein-Protein	8 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	2 [view]
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0019869	chloride channel inhibitor activity

Biological Process

GO:0006468	protein phosphorylation
GO:0010765	positive regulation of sodium ion transport
GO:0010800	positive regulation of peptidyl-threonine phosphorylation
GO:0032414	positive regulation of ion transmembrane transporter activity
GO:0035556	intracellular signal transduction
GO:0043066	negative regulation of apoptotic process
GO:0046777	protein autophosphorylation
GO:0051928	positive regulation of calcium ion transport
GO:0090004	positive regulation of establishment of protein localization to plasma membrane
GO:2000021	regulation of ion homeostasis
GO:2000651	positive regulation of sodium ion transmembrane transporter activity
GO:2000682	positive regulation of rubidium ion transport
GO:2000688	positive regulation of rubidium ion transmembrane transporter activity

Cellular Component

GO:0005737	cytoplasm
GO:0005912	adherens junction
GO:0005923	tight junction

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain
IPR024678 Serine/threonine-protein kinase OSR1/WNK, CCT domain

PFAM

PF00069
PF07714
PF12202

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt