InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RNF4

Summary

InnateDB Protein

IDBP-7541.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RNF4

Protein Name

ring finger protein 4

Synonyms

SLX5; SNURF;

Species

Homo sapiens

Ensembl Protein

ENSP00000315212

InnateDB Gene

IDBG-7539 (RNF4)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation. {ECO:0000269PubMed:12885770, ECO:0000269PubMed:18408734, ECO:0000269PubMed:19307308, ECO:0000269PubMed:20026589, ECO:0000269PubMed:20212317, ECO:0000269PubMed:20943951}.

Subcellular Localization

Cytoplasm. Nucleus. Nucleus, PML body. Nucleus, nucleoplasm.

Disease Associations

Tissue Specificity

Widely expressed at low levels in many tissues; highly expressed in testis. {ECO:0000269PubMed:9479498}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 65 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	65 [view]
Protein-Protein	62 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0003713	transcription coactivator activity
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0031491	nucleosome binding
GO:0032184	SUMO polymer binding
GO:0046872	metal ion binding
GO:0050681	androgen receptor binding

Biological Process

GO:0006351	transcription, DNA-templated
GO:0030521	androgen receptor signaling pathway
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0045893	positive regulation of transcription, DNA-templated
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0046685	response to arsenic-containing substance
GO:0051865	protein autoubiquitination
GO:0070534	protein K63-linked ubiquitination
GO:0070936	protein K48-linked ubiquitination
GO:0070979	protein K11-linked ubiquitination
GO:0085020	protein K6-linked ubiquitination
GO:0090169	regulation of spindle assembly
GO:0090234	regulation of kinetochore assembly