InnateDB Protein
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IDBP-75621.6
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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NONO
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Protein Name
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non-POU domain containing, octamer-binding
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Synonyms
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NMT55; NRB54; P54; P54NRB; PPP1R114;
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Species
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Homo sapiens
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Ensembl Protein
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ENSP00000276079
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InnateDB Gene
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IDBG-75619 (NONO)
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Protein Structure
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Function |
DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP- dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. {ECO:0000269PubMed:10858305, ECO:0000269PubMed:11525732, ECO:0000269PubMed:11897684, ECO:0000269PubMed:15590677, ECO:0000269PubMed:22416126}.
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Subcellular Localization |
Nucleus. Nucleus, nucleolus. Nucleus speckle. Note=Detected in punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles.
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Disease Associations |
Note=A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.
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Tissue Specificity |
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines. {ECO:0000269PubMed:9341872}.
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 157 experimentally validated interaction(s) in this database.
They are also associated with 12 interaction(s) predicted by orthology.
Experimentally validated |
Total |
157
[view]
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Protein-Protein |
155
[view]
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Protein-DNA |
2
[view]
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Predicted by orthology |
Total |
12 [view]
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Molecular Function |
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Biological Process |
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Cellular Component |
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PDB ID |
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InterPro |
IPR000504
RNA recognition motif domain
IPR012975
NOPS
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PFAM |
PF00076
PF08075
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PRINTS |
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PIRSF |
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SMART |
SM00360
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TIGRFAMs |
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Modification |
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SwissProt |
Q15233
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PhosphoSite |
PhosphoSite-Q15233
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TrEMBL |
C9JJ13
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UniProt Splice Variant |
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Entrez Gene |
4841
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UniGene |
Hs.572371
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RefSeq |
NP_031389
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HUGO |
HGNC:7871
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OMIM |
300084
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CCDS |
CCDS14410
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HPRD |
02098
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IMGT |
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EMBL |
AK297144
AL590762
BC002364
BC003129
BC012141
BC028299
BC069616
BC069639
CH471132
CR456761
L14599
U02493
U89867
Y11289
Y11290
Y11291
Y11292
Y11293
Y11294
Y11295
Y11296
Y11297
Y11298
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GenPept |
AAA03427
AAC37578
AAC51852
AAH02364
AAH03129
AAH12141
AAH28299
AAH69616
AAH69639
BAH12508
CAA72157
CAG33042
EAX05298
EAX05299
EAX05300
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