InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: NONO

Summary

InnateDB Protein

IDBP-75623.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NONO

Protein Name

non-POU domain containing, octamer-binding

Synonyms

NMT55; NRB54; P54; P54NRB; PPP1R114;

Species

Homo sapiens

Ensembl Protein

ENSP00000362963

InnateDB Gene

IDBG-75619 (NONO)

Protein Structure

UniProt Annotation

Function

DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP- dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. {ECO:0000269PubMed:10858305, ECO:0000269PubMed:11525732, ECO:0000269PubMed:11897684, ECO:0000269PubMed:15590677, ECO:0000269PubMed:22416126}.

Subcellular Localization

Nucleus. Nucleus, nucleolus. Nucleus speckle. Note=Detected in punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles.

Disease Associations

Note=A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.

Tissue Specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines. {ECO:0000269PubMed:9341872}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 157 experimentally validated interaction(s) in this database.
They are also associated with 12 interaction(s) predicted by orthology.

Experimentally validated
Total	157 [view]
Protein-Protein	155 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	12 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0001047	core promoter binding
GO:0003676	nucleic acid binding
GO:0005515	protein binding
GO:0042802	identical protein binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0006281	DNA repair
GO:0006310	DNA recombination
GO:0006351	transcription, DNA-templated
GO:0006397	mRNA processing
GO:0007623	circadian rhythm
GO:0008380	RNA splicing
GO:0042752	regulation of circadian rhythm
GO:0045892	negative regulation of transcription, DNA-templated