InnateDB Protein
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IDBP-763799.3
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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SUPT4H1
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Protein Name
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Synonyms
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SPT4; SPT4H; SUPT4H;
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Species
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Homo sapiens
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Ensembl Protein
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ENSP00000462670
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InnateDB Gene
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IDBG-244315 (SUPT4H1)
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Protein Structure
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Function |
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV- 1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences. {ECO:0000269PubMed:10075709, ECO:0000269PubMed:10199401, ECO:0000269PubMed:10454543, ECO:0000269PubMed:10912001, ECO:0000269PubMed:11112772, ECO:0000269PubMed:11553615, ECO:0000269PubMed:12653964, ECO:0000269PubMed:12718890, ECO:0000269PubMed:15136722, ECO:0000269PubMed:15380072, ECO:0000269PubMed:16214896, ECO:0000269PubMed:9450929, ECO:0000269PubMed:9857195}.
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Subcellular Localization |
Nucleus {ECO:0000269PubMed:8649394}.
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Disease Associations |
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Tissue Specificity |
Widely expressed. {ECO:0000269PubMed:8649394, ECO:0000269PubMed:8786137}.
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 16 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.
Experimentally validated |
Total |
16
[view]
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Protein-Protein |
15
[view]
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Protein-DNA |
1
[view]
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Predicted by orthology |
Total |
2 [view]
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Molecular Function |
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Biological Process |
GO:0000122
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negative regulation of transcription from RNA polymerase II promoter
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GO:0006338
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chromatin remodeling
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GO:0006355
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regulation of transcription, DNA-templated
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GO:0006357
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regulation of transcription from RNA polymerase II promoter
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GO:0006366
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transcription from RNA polymerase II promoter
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GO:0006368
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transcription elongation from RNA polymerase II promoter
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GO:0010467
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gene expression
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GO:0016032
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viral process
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GO:0032785
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negative regulation of DNA-dependent transcription, elongation
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GO:0032786
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positive regulation of DNA-templated transcription, elongation
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GO:0045944
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positive regulation of transcription from RNA polymerase II promoter
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GO:0050434
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positive regulation of viral transcription
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Cellular Component |
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PDB ID |
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InterPro |
IPR009287
Transcription initiation Spt4
IPR022800
Spt4/RpoE2 zinc finger
IPR029040
RNA polymerase subunit RPABC4/transcription elongation factor Spt4
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PFAM |
PF06093
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PRINTS |
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PIRSF |
PIRSF025023
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SMART |
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TIGRFAMs |
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Modification |
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SwissProt |
P63272
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PhosphoSite |
PhosphoSite-P63272
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TrEMBL |
J3QR04
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UniProt Splice Variant |
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Entrez Gene |
6827
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UniGene |
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RefSeq |
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HUGO |
HGNC:11467
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OMIM |
603555
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CCDS |
CCDS11606
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HPRD |
16026
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IMGT |
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EMBL |
AC004687
AK129758
AK311986
BC002802
CH471109
CR407663
U38817
U38818
U43923
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GenPept |
AAB07814
AAB18674
AAB18675
AAH02802
BAC85230
BAG34925
CAG28591
EAW94464
EAW94465
EAW94466
EAW94467
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