InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: WNK2

Summary

InnateDB Protein

IDBP-76534.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

WNK2

Protein Name

WNK lysine deficient protein kinase 2

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000297954

InnateDB Gene

IDBG-76524 (WNK2)

Protein Structure

UniProt Annotation

Function

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity. {ECO:0000269PubMed:17667937, ECO:0000269PubMed:18593598, ECO:0000269PubMed:21733846}.

Subcellular Localization

Cytoplasm. Cell membrane.

Disease Associations

Tissue Specificity

Expressed in various cancer cell lines (at protein level). Predominantly expressed in heart, brain, skeletal muscle and colon. {ECO:0000269PubMed:11571656, ECO:0000269PubMed:17667937, ECO:0000269PubMed:21733846}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 8 experimentally validated interaction(s) in this database.

Experimentally validated
Total	8 [view]
Protein-Protein	8 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups

Biological Process

GO:0006468	protein phosphorylation
GO:0008285	negative regulation of cell proliferation
GO:0035556	intracellular signal transduction
GO:0046777	protein autophosphorylation
GO:0070373	negative regulation of ERK1 and ERK2 cascade
GO:2000021	regulation of ion homeostasis
GO:2000651	positive regulation of sodium ion transmembrane transporter activity

Cellular Component

GO:0005737	cytoplasm
GO:0005886	plasma membrane

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain
IPR024678 Serine/threonine-protein kinase OSR1/WNK, CCT domain

PFAM

PF00069
PF07714
PF12202

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt