InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RAD23B

Summary

InnateDB Protein

IDBP-79799.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RAD23B

Protein Name

RAD23 homolog B (S. cerevisiae)

Synonyms

HHR23B; HR23B; P58;

Species

Homo sapiens

Ensembl Protein

ENSP00000350708

InnateDB Gene

IDBG-79797 (RAD23B)

Protein Structure

UniProt Annotation

Function

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum- associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation.The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.

Subcellular Localization

Nucleus. Cytoplasm. Note=The intracellular distribution is cell cycle dependent. Localized to the nucleus and the cytoplasm during G1 phase. Nuclear levels decrease during S- phase; upon entering mitosis, relocalizes in the cytoplasm without association with chromatin.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 248 experimentally validated interaction(s) in this database.
They are also associated with 9 interaction(s) predicted by orthology.

Experimentally validated
Total	248 [view]
Protein-Protein	247 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	9 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003684	damaged DNA binding
GO:0003697	single-stranded DNA binding
GO:0005515	protein binding
GO:0031593	polyubiquitin binding

Biological Process

GO:0000715	nucleotide-excision repair, DNA damage recognition
GO:0000718	nucleotide-excision repair, DNA damage removal
GO:0006281	DNA repair
GO:0006289	nucleotide-excision repair
GO:0006974	cellular response to DNA damage stimulus
GO:0007283	spermatogenesis
GO:0032434	regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process

Cellular Component

GO:0000502	proteasome complex
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0071942	XPC complex

Protein Structure and Domains

PDB ID

InterPro

IPR000449 Ubiquitin-associated domain/translation elongation factor EF-Ts, N-terminal
IPR000626 Ubiquitin-like
IPR004806 UV excision repair protein Rad23
IPR006636 Heat shock chaperonin-binding
IPR009060 UBA-like
IPR015360 XPC-binding domain
IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote
IPR022617 Rad60/SUMO-like domain
IPR029071 Ubiquitin-related domain

PFAM

PF00627
PF00240
PF14560
PF09280
PF11976

PRINTS

PR01839

PIRSF

SMART

SM00213
SM00727
SM00165

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt