InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: MOCS3

Summary

InnateDB Protein

IDBP-81187.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

MOCS3

Protein Name

molybdenum cofactor synthesis 3

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000244051

InnateDB Gene

IDBG-81183 (MOCS3)

Protein Structure

UniProt Annotation

Function

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. {ECO:0000255HAMAP-Rule:MF_03049, ECO:0000269PubMed:15073332, ECO:0000269PubMed:19017811}.

Subcellular Localization

Cytoplasm {ECO:0000255HAMAP-Rule:MF_03049, ECO:0000269PubMed:15073332}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 8 experimentally validated interaction(s) in this database.

Experimentally validated
Total	8 [view]
Protein-Protein	7 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003824	catalytic activity
GO:0004792	thiosulfate sulfurtransferase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016779	nucleotidyltransferase activity
GO:0016783	sulfurtransferase activity
GO:0042292	URM1 activating enzyme activity
GO:0046872	metal ion binding
GO:0061604	molybdopterin-synthase sulfurtransferase activity
GO:0061605	molybdopterin-synthase adenylyltransferase activity
GO:0070733	protein adenylyltransferase activity

Biological Process

GO:0002098	tRNA wobble uridine modification
GO:0002143	tRNA wobble position uridine thiolation
GO:0006766	vitamin metabolic process
GO:0006767	water-soluble vitamin metabolic process
GO:0006777	Mo-molybdopterin cofactor biosynthetic process
GO:0018117	protein adenylylation
GO:0018192	enzyme active site formation via L-cysteine persulfide
GO:0032324	molybdopterin cofactor biosynthetic process
GO:0032447	protein urmylation
GO:0034227	tRNA thio-modification
GO:0044281	small molecule metabolic process