InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: NEK6

Summary

InnateDB Protein

IDBP-84708.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NEK6

Protein Name

NIMA (never in mitosis gene a)-related kinase 6

Synonyms

SID6-1512;

Species

Homo sapiens

Ensembl Protein

ENSP00000319734

InnateDB Gene

IDBG-84700 (NEK6)

Protein Structure

UniProt Annotation

Function

Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. {ECO:0000269PubMed:12054534, ECO:0000269PubMed:14563848, ECO:0000269PubMed:18728393, ECO:0000269PubMed:19001501, ECO:0000269PubMed:19414596, ECO:0000269PubMed:20407017, ECO:0000269PubMed:20873783, ECO:0000269PubMed:21099361}.

Subcellular Localization

Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Colocalizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis.

Disease Associations

Tissue Specificity

Ubiquitous, with highest expression in heart and skeletal muscle. Up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers. {ECO:0000269PubMed:12054534, ECO:0000269PubMed:20407017}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 56 experimentally validated interaction(s) in this database.

Experimentally validated
Total	56 [view]
Protein-Protein	55 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000287	magnesium ion binding
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0004871	signal transducer activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0019894	kinesin binding
GO:0019901	protein kinase binding

Biological Process

GO:0000278	mitotic cell cycle
GO:0000910	cytokinesis
GO:0006468	protein phosphorylation
GO:0006915	apoptotic process
GO:0007059	chromosome segregation
GO:0007067	mitotic nuclear division
GO:0007077	mitotic nuclear envelope disassembly
GO:0007165	signal transduction
GO:0007346	regulation of mitotic cell cycle
GO:0018105	peptidyl-serine phosphorylation
GO:0030071	regulation of mitotic metaphase/anaphase transition
GO:0031572	G2 DNA damage checkpoint
GO:0043123	positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0046777	protein autophosphorylation
GO:0051225	spindle assembly
GO:2000772	regulation of cellular senescence

Cellular Component

GO:0000922	spindle pole
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005815	microtubule organizing center
GO:0005829	cytosol
GO:0005874	microtubule
GO:0016607	nuclear speck

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF07714

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt