InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: XPO5

Summary

InnateDB Protein

IDBP-88430.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

XPO5

Protein Name

exportin 5

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000265351

InnateDB Gene

IDBG-88428 (XPO5)

Protein Structure

UniProt Annotation

Function

Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner.Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference, and adenovirus VA1 dsRNA. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence- independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.

Subcellular Localization

Nucleus {ECO:0000269PubMed:11777942}. Cytoplasm {ECO:0000269PubMed:11777942}. Note=Shuttles between the nucleus and the cytoplasm.

Disease Associations

Tissue Specificity

Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. {ECO:0000269PubMed:11777942}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 61 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	61 [view]
Protein-Protein	61 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000049	tRNA binding
GO:0003723	RNA binding
GO:0005488	binding
GO:0005515	protein binding
GO:0008536	Ran GTPase binding
GO:0008565	protein transporter activity
GO:0044822	poly(A) RNA binding