InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: SPEN

Summary

InnateDB Protein

IDBP-91163.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

SPEN

Protein Name

spen homolog, transcriptional regulator (Drosophila)

Synonyms

HIAA0929; MINT; RBM15C; SHARP;

Species

Homo sapiens

Ensembl Protein

ENSP00000364912

InnateDB Gene

IDBG-91161 (SPEN)

Protein Structure

UniProt Annotation

Function

May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2- mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA. {ECO:0000250, ECO:0000269PubMed:11331609, ECO:0000269PubMed:12374742}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:11331609}. Note=Associates with chromatin.

Disease Associations

Tissue Specificity

Expressed at high level in brain, testis, spleen and thymus. Expressed at intermediate level in kidney, liver, mammary gland and skin.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 44 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	44 [view]
Protein-Protein	43 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0001085	RNA polymerase II transcription factor binding
GO:0001191	RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription
GO:0003676	nucleic acid binding
GO:0003697	single-stranded DNA binding
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0003714	transcription corepressor activity
GO:0005515	protein binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006351	transcription, DNA-templated
GO:0007219	Notch signaling pathway
GO:0016032	viral process
GO:0045892	negative regulation of transcription, DNA-templated
GO:0045893	positive regulation of transcription, DNA-templated
GO:0050769	positive regulation of neurogenesis

Cellular Component

GO:0005634	nucleus
GO:0017053	transcriptional repressor complex
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

InterPro

IPR000504 RNA recognition motif domain
IPR010912 Spen paralogue/orthologue C-terminal, metazoa
IPR012921 Spen paralogue and orthologue SPOC, C-terminal
IPR016194 SPOC like C-terminal domain

PFAM

PF00076
PF07744

PRINTS

PIRSF

SMART

SM00360

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q96T58