InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: LIN28B

Summary

InnateDB Protein

IDBP-94773.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

LIN28B

Protein Name

lin-28 homolog B (C. elegans)

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000344401

InnateDB Gene

IDBG-94771 (LIN28B)

Protein Structure

UniProt Annotation

Function

Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth. {ECO:0000269PubMed:16971064, ECO:0000269PubMed:18951094, ECO:0000269PubMed:19703396}.

Subcellular Localization

Cytoplasm. Nucleus. Note=Predominantly cytoplasmic at G1 phase, accumulates in the nucleus in S and G2 phases. The frequency of nuclear localization in S and in G2 phases is 60% and 30%, respectively.

Disease Associations

Tissue Specificity

High expression in testis, fetal liver, placenta and in hepatocellular carcinoma (HCC). Isoform 1 is only detected in moderately and poorly differentiated HCC tissues and placenta (at protein level). Isoform 2 is detected in fetal liver, non-tumor liver tissues, as well as well-differentiated tumor tissues (at protein level). {ECO:0000269PubMed:16971064}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 28 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	28 [view]
Protein-Protein	28 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0044822	poly(A) RNA binding