InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PPP1R8

Summary

InnateDB Protein

IDBP-95033.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PPP1R8

Protein Name

protein phosphatase 1, regulatory subunit 8

Synonyms

ARD-1; ARD1; NIPP-1; NIPP1; PRO2047;

Species

Homo sapiens

Ensembl Protein

ENSP00000363042

InnateDB Gene

IDBG-95029 (PPP1R8)

Protein Structure

UniProt Annotation

Function

Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.

Subcellular Localization

Nucleus. Nucleus speckle. Note=Primarily, but not exclusively, nuclear.Isoform Gamma: Cytoplasm. Note=Found mainly in the cytoplasm.

Disease Associations

Tissue Specificity

Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells. {ECO:0000269PubMed:10103062, ECO:0000269PubMed:7499293}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 44 experimentally validated interaction(s) in this database.

Experimentally validated
Total	44 [view]
Protein-Protein	43 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0004865	protein serine/threonine phosphatase inhibitor activity
GO:0005515	protein binding
GO:0008995	ribonuclease E activity

Biological Process

GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0006397	mRNA processing
GO:0006401	RNA catabolic process
GO:0008380	RNA splicing
GO:0043086	negative regulation of catalytic activity
GO:0090501	RNA phosphodiester bond hydrolysis