InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: MACF1

Summary

InnateDB Protein

IDBP-96628.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

MACF1

Protein Name

microtubule-actin crosslinking factor 1

Synonyms

ABP620; ACF7; MACF; OFC4;

Species

Homo sapiens

Ensembl Protein

ENSP00000289893

InnateDB Gene

IDBG-96610 (MACF1)

Protein Structure

UniProt Annotation

Function

Isoform 2 is a F-actin-binding protein which may play a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules. Plays an important role in ERBB2- dependent stabilization of microtubules at the cell cortex. Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane. Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics. May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4. Plays a key role in wound healing and epidermal cell migration. Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate MT dynamics and polarize hair follicle stem cells (By similarity). {ECO:0000250}.

Subcellular Localization

Isoform 2: Cytoplasm, cytoskeleton. Cytoplasm. Golgi apparatus. Cell membrane. Cell projection, ruffle membrane. Note=The phosphorylated form is found in the cytoplasm while the non-phosphorylated form associates with the microtubules (By similarity). Localizes to the tips of microtubules. APC controls its localization to the cell membrane which is critical for its function in microtubule stabilization. {ECO:0000250}.Isoform 1: Cytoplasm. Golgi apparatus. Note=Localizes to the tips of microtubules.

Disease Associations

Tissue Specificity

Isoform 2 is ubiquitously expressed. Isoform 1 is expressed in cell lines NCI-H460, A-549 and HaCaT. Isoform 4 is expressed in heart, lung, pituitary and placenta, not found in brain, kidney, liver, pancreas or skeletal muscle. {ECO:0000269PubMed:11845288, ECO:0000269PubMed:16076900}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 43 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.

Experimentally validated
Total	43 [view]
Protein-Protein	43 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	1 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003779	actin binding
GO:0005509	calcium ion binding
GO:0005515	protein binding
GO:0016887	ATPase activity
GO:0044822	poly(A) RNA binding
GO:0051015	actin filament binding

Biological Process

GO:0006200	ATP catabolic process
GO:0007050	cell cycle arrest
GO:0010632	regulation of epithelial cell migration
GO:0030177	positive regulation of Wnt signaling pathway
GO:0032886	regulation of microtubule-based process
GO:0042060	wound healing
GO:0043001	Golgi to plasma membrane protein transport
GO:0051893	regulation of focal adhesion assembly

Cellular Component

GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0005856	cytoskeleton
GO:0005874	microtubule
GO:0005886	plasma membrane
GO:0032587	ruffle membrane

Protein Structure and Domains

PDB ID

InterPro

IPR001101 Plectin repeat
IPR002017 Spectrin repeat
IPR002048 EF-hand domain
IPR003108 Growth-arrest-specific protein 2 domain
IPR018159 Spectrin/alpha-actinin

PFAM

PF00681
PF00435
PF00036
PF13202
PF13405
PF02187

PRINTS

PIRSF

SMART

SM00250
SM00054
SM00243
SM00150

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9UPN3