Homo sapiens Gene: IFI16
Summary
InnateDB Gene IDBG-103852.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol IFI16
Gene Name interferon, gamma-inducible protein 16
Synonyms IFNGIP1; PYHIN2
Species Homo sapiens
Ensembl Gene ENSG00000163565
Encoded Proteins
interferon, gamma-inducible protein 16
interferon, gamma-inducible protein 16
interferon, gamma-inducible protein 16
interferon, gamma-inducible protein 16
interferon, gamma-inducible protein 16
interferon, gamma-inducible protein 16
interferon, gamma-inducible protein 16
interferon, gamma-inducible protein 16
Protein Structure
Useful resources Stemformatics EHFPI ImmGen
InnateDB Annotation
Summary
IFI16, a PYHIN protein, is an intracellular DNA sensor that mediates the induction of interferon-beta (IFNB) by directly associating with IFNB-inducing viral DNA motifs.
IFI16 acts as a nuclear pathogen sensor and interacts with PYCARD and CASP1 to form a functional inflammasome during KSHV infections.
Cyclic-di-GMP-induced levels of IFI16 suppress the expression of TMEM173 (STING).
IFI16 is essential for host defence by clustering into signalling foci with foreign DNA in a switch-like manner and is capable of using the size of naked double stranded DNA as a molecular ruler to distinguish self from nonself.
In herpes simplex virus 1 (HSV-1) infected cells, the stability and function of IFI16 and TMEM173 are dependent on cell derivation and the functional integrity of HSV-1 proteins ICP0 and US3 protein kinase.
IFI16 is a sensor for lentiviral reverse transcription products and restricts HIV-1 replication in macrophages.
IFI16 oligomerizes upon viral DNA sensing in human cytomegalovirus (HCMV) infected-cells. The HCMV major tegument protein pUL83 blocks nuclear IFI16 oligomerization and inhibits IFI16-mediated antiviral cytokine expression
The end result of the interplay between TMEM173 (STING), IFI16, and herpes simplex virus 1 (HSV-1) is determined by the genotype of the infected cells and the functional integrity of HSV-1 proteins infected cell protein 0 (ICP0) and US3 protein kinase.
IFI16 is a sensor for lentiviral reverse transcription products and restricts HIV-1 replication in human macrophages.
IFI16 transcriptionally regulates type-I interferons and DDX58 (RIG-I) and controls the interferon response to both DNA and RNA viruses.
IFI16 and MB21D1 interact and cooperate during herpes simplex virus infection to initiate innate signalling.
IFI16 restricts chromatinized human papillomaviruses DNA through epigenetic modifications, thus reducing both viral replication and transcription.
Single nucleotide polymorphisms in IFI16 and AIM2 are associated with Beh├žet disease.
Entrez Gene
Summary This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]
Gene Information
Type Protein coding
Genomic Location Chromosome 1:158999968-159055155
Strand Forward strand
Band q23.1
Transcripts
ENST00000359709 ENSP00000352740
ENST00000368132 ENSP00000357114
ENST00000368131 ENSP00000357113
ENST00000295809 ENSP00000295809
ENST00000340979 ENSP00000342741
ENST00000426592 ENSP00000406406
ENST00000447473 ENSP00000407052
ENST00000448393 ENSP00000404325
ENST00000493884
ENST00000483916
ENST00000474473 ENSP00000456129
ENST00000562225
ENST00000566111 ENSP00000458084
ENST00000567661 ENSP00000456094
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 23 experimentally validated interaction(s) in this database.
Experimentally validated
Total 23 [view]
Protein-Protein 20 [view]
Protein-DNA 3 [view]
Protein-RNA 0
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Gene Ontology

Molecular Function
Accession GO Term
GO:0003690 double-stranded DNA binding
GO:0005515 protein binding
GO:0008134 transcription factor binding
GO:0044822 poly(A) RNA binding
Biological Process
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0001819 positive regulation of cytokine production
GO:0002218 activation of innate immune response
GO:0006351 transcription, DNA-templated
GO:0006914 autophagy
GO:0006954 inflammatory response
GO:0008283 cell proliferation
GO:0010506 regulation of autophagy
GO:0030097 hemopoiesis
GO:0030099 myeloid cell differentiation
GO:0030224 monocyte differentiation
GO:0032481 positive regulation of type I interferon production
GO:0032731 positive regulation of interleukin-1 beta production
GO:0042149 cellular response to glucose starvation
GO:0042771 intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
GO:0043392 negative regulation of DNA binding
GO:0045071 negative regulation of viral genome replication
GO:0045087 innate immune response (InnateDB)
GO:0045824 negative regulation of innate immune response
GO:0045892 negative regulation of transcription, DNA-templated
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0051607 defense response to virus
GO:0071479 cellular response to ionizing radiation
GO:0072332 intrinsic apoptotic signaling pathway by p53 class mediator
GO:0097202 activation of cysteine-type endopeptidase activity
GO:2000117 negative regulation of cysteine-type endopeptidase activity
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016020 membrane
GO:0016607 nuclear speck
Orthologs
No orthologs found for this gene
Pathways
NETPATH
ID pathway
REACTOME
STING mediated induction of host immune responses pathway
IRF3-mediated induction of type I IFN pathway
Innate Immune System pathway
Immune System pathway
Cytosolic sensors of pathogen-associated DNA pathway
KEGG
INOH
PID NCI
Cross-References
SwissProt
TrEMBL H3BM18 H3BVE6
UniProt Splice Variant
Entrez Gene 3428
UniGene Hs.380250 Hs.629190
RefSeq NM_001206567 NM_005531 XM_005245127 XM_006711290
HUGO HGNC:5395
OMIM 147586
CCDS CCDS1180 CCDS58039
HPRD 00969
IMGT
EMBL AL359753
GenPept
RNA Seq Atlas 3428