InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PKN2

Summary

InnateDB Protein

IDBP-100131.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PKN2

Protein Name

protein kinase N2

Synonyms

Pak-2; PAK2; PRK2; PRKCL2; PRO2042; STK7;

Species

Homo sapiens

Ensembl Protein

ENSP00000359552

InnateDB Gene

IDBG-100129 (PKN2)

Protein Structure

UniProt Annotation

Function

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c- fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication. {ECO:0000269PubMed:10226025, ECO:0000269PubMed:10926925, ECO:0000269PubMed:11777936, ECO:0000269PubMed:11781095, ECO:0000269PubMed:15364941, ECO:0000269PubMed:17332740, ECO:0000269PubMed:20188095, ECO:0000269PubMed:20974804, ECO:0000269PubMed:21754995, ECO:0000269PubMed:9121475}.

Subcellular Localization

Cytoplasm. Nucleus. Membrane {ECO:0000250}. Cell projection, lamellipodium. Cytoplasm, cytoskeleton. Cleavage furrow. Midbody. Cell junction. Note=Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm.

Disease Associations

Tissue Specificity

Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells. {ECO:0000269PubMed:10026169, ECO:0000269PubMed:21754995}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 49 experimentally validated interaction(s) in this database.

Experimentally validated
Total	49 [view]
Protein-Protein	44 [view]
Protein-DNA	4 [view]
Protein-RNA	1 [view]
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004697	protein kinase C activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0042826	histone deacetylase binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0006468	protein phosphorylation
GO:0006915	apoptotic process
GO:0007049	cell cycle
GO:0007155	cell adhesion
GO:0007165	signal transduction
GO:0010631	epithelial cell migration
GO:0032467	positive regulation of cytokinesis
GO:0043297	apical junction assembly
GO:0045931	positive regulation of mitotic cell cycle
GO:0051301	cell division
GO:2000145	regulation of cell motility

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005813	centrosome
GO:0016020	membrane
GO:0030027	lamellipodium
GO:0030496	midbody
GO:0032154	cleavage furrow
GO:0043296	apical junction complex
GO:0045111	intermediate filament cytoskeleton

Protein Structure and Domains

PDB ID

InterPro

IPR000008 C2 domain
IPR000719 Protein kinase domain
IPR000961 AGC-kinase, C-terminal
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR011072 HR1 rho-binding repeat
IPR017892 Protein kinase, C-terminal
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00168
PF00069
PF07714
PF02185
PF00433

PRINTS

PR00360
PR00109

PIRSF

SMART

SM00239
SM00133
SM00220
SM00742
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt