InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CLK2

Summary

InnateDB Protein

IDBP-103226.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CLK2

Protein Name

CDC-like kinase 2

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000357345

InnateDB Gene

IDBG-103224 (CLK2)

Protein Structure

UniProt Annotation

Function

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. {ECO:0000269PubMed:10480872, ECO:0000269PubMed:19168442, ECO:0000269PubMed:8910305, ECO:0000269PubMed:9637771}.

Subcellular Localization

Isoform 1: Nucleus. Nucleus speckle. Note=Inhibition of phosphorylation at Ser-142 results in accumulation in the nuclear speckle. {ECO:0000250}.Isoform 2: Nucleus speckle. Note=Co- localizes with serine- and arginine-rich (SR) proteins in the nuclear speckles.

Disease Associations

Tissue Specificity

Endothelial cells. {ECO:0000269PubMed:19168442}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 47 experimentally validated interaction(s) in this database.

Experimentally validated
Total	47 [view]
Protein-Protein	47 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004712	protein serine/threonine/tyrosine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups

Biological Process

GO:0006468	protein phosphorylation
GO:0010033	response to organic substance
GO:0010212	response to ionizing radiation
GO:0018108	peptidyl-tyrosine phosphorylation
GO:0032526	response to retinoic acid
GO:0043484	regulation of RNA splicing
GO:0045721	negative regulation of gluconeogenesis
GO:0046777	protein autophosphorylation

Cellular Component

GO:0005634	nucleus
GO:0016607	nuclear speck

Protein Structure and Domains

PDB ID

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF07714

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt