InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CENPF

Summary

InnateDB Protein

IDBP-106650.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CENPF

Protein Name

centromere protein F, 350/400kDa (mitosin)

Synonyms

CENF; hcp-1; PRO1779;

Species

Homo sapiens

Ensembl Protein

ENSP00000355922

InnateDB Gene

IDBG-106648 (CENPF)

Protein Structure

UniProt Annotation

Function

Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia. {ECO:0000269PubMed:12974617, ECO:0000269PubMed:17600710, ECO:0000269PubMed:7542657, ECO:0000269PubMed:7651420}.

Subcellular Localization

Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 25 experimentally validated interaction(s) in this database.

Experimentally validated
Total	25 [view]
Protein-Protein	22 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003682	chromatin binding
GO:0005515	protein binding
GO:0008022	protein C-terminus binding
GO:0008134	transcription factor binding
GO:0042803	protein homodimerization activity
GO:0045502	dynein binding

Biological Process

GO:0000085	mitotic G2 phase
GO:0000086	G2/M transition of mitotic cell cycle
GO:0000087	mitotic M phase
GO:0000278	mitotic cell cycle
GO:0006260	DNA replication
GO:0007059	chromosome segregation
GO:0007067	mitotic nuclear division
GO:0007094	mitotic spindle assembly checkpoint
GO:0007517	muscle organ development
GO:0008283	cell proliferation
GO:0010389	regulation of G2/M transition of mitotic cell cycle
GO:0015031	protein transport
GO:0016202	regulation of striated muscle tissue development
GO:0030154	cell differentiation
GO:0042493	response to drug
GO:0045892	negative regulation of transcription, DNA-templated
GO:0051310	metaphase plate congression
GO:0051382	kinetochore assembly
GO:0051726	regulation of cell cycle

Cellular Component

GO:0000775	chromosome, centromeric region
GO:0000776	kinetochore
GO:0000785	chromatin
GO:0000922	spindle pole
GO:0000940	condensed chromosome outer kinetochore
GO:0005634	nucleus
GO:0005635	nuclear envelope
GO:0005737	cytoplasm
GO:0005819	spindle
GO:0005829	cytosol
GO:0016363	nuclear matrix
GO:0030496	midbody
GO:0045120	pronucleus
GO:0048471	perinuclear region of cytoplasm

Protein Structure and Domains

PDB ID

InterPro

IPR018302 Kinetochore protein Cenp-F/LEK1, Rb protein-binding domain
IPR018463 Centromere protein Cenp-F, N-terminal
IPR019513 Centromere protein Cenp-F, leucine-rich repeat-containing domain

PFAM

PF10490
PF10481
PF10473

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P49454

PhosphoSite