InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Epm2a

Summary

InnateDB Protein

IDBP-133618.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Epm2a

Protein Name

epilepsy, progressive myoclonic epilepsy, type 2 gene alpha

Synonyms

TG-B; Tg(TcraK,TcrbK)TG-BFlv;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000066050

InnateDB Gene

IDBG-133616 (Epm2a)

Protein Structure

UniProt Annotation

Function

Has been shown to have both dual-specificity protein phosphatase and glucan phosphatase activities and, together with the E3 ubiquitin ligase NHLRC1/malin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. Dephosphorylates phosphotyrosine, phosphoserine and phosphothreonine substrates in vitro. Has also been shown to dephosphorylate MAPT. Shows strong phosphatase activity towards complex carbohydrates in vitro, avoiding glycogen hyperphosphorylation which is associated with reduced branching and formation of insoluble aggregates. Forms a complex with NHLRC1/malin and HSP70, which suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Acts as a scaffold protein to facilitate PPP1R3C/PTG ubiquitination by NHLRC1/malin. Also promotes proteasome-independent protein degradation through the macroautophagy pathway. {ECO:0000269PubMed:18040046, ECO:0000269PubMed:18852261, ECO:0000269PubMed:19036738, ECO:0000269PubMed:19542233, ECO:0000269PubMed:20453062, ECO:0000269PubMed:22669944}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:15102711}. Endoplasmic reticulum {ECO:0000269PubMed:15102711}. Note=Under glycogenolytic conditions localizes to the nucleus. {ECO:0000250}.

Disease Associations

Tissue Specificity

Widely expressed. Higher levels of expression are found in heart, brain, liver, skeletal muscle and kidney. Found in neuronal dendrites and perikarya, but not in axons. {ECO:0000269PubMed:10092504, ECO:0000269PubMed:15102711}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 11 experimentally validated interaction(s) in this database.
They are also associated with 14 interaction(s) predicted by orthology.

Experimentally validated
Total	11 [view]
Protein-Protein	11 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	14 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004439	phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity
GO:0004725	protein tyrosine phosphatase activity
GO:0005515	protein binding
GO:0008138	protein tyrosine/serine/threonine phosphatase activity
GO:0016791	phosphatase activity
GO:0019203	carbohydrate phosphatase activity
GO:0030246	carbohydrate binding
GO:2001070	starch binding

Biological Process

GO:0005977	glycogen metabolic process
GO:0006470	protein dephosphorylation
GO:0006914	autophagy
GO:0007399	nervous system development
GO:0016311	dephosphorylation
GO:0035335	peptidyl-tyrosine dephosphorylation
GO:0046855	inositol phosphate dephosphorylation
GO:0046959	habituation

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005783	endoplasmic reticulum
GO:0005829	cytosol
GO:0005844	polysome

Protein Structure and Domains

PDB ID

MGI:1341085

InterPro

IPR000340 Dual specificity phosphatase, catalytic domain
IPR000387 Protein-tyrosine/Dual specificity phosphatase
IPR002044 Carbohydrate binding module family 20
IPR013784 Carbohydrate-binding-like fold
IPR020422 Dual specificity phosphatase, subgroup, catalytic domain
IPR029021 Protein-tyrosine phosphatase-like

PFAM

PF00782
PF00686

PRINTS

PIRSF

SMART

SM01065
SM00195

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9WUA5