InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Eif3e

Summary

InnateDB Protein

IDBP-137967.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Eif3e

Protein Name

eukaryotic translation initiation factor 3, subunit E

Synonyms

48kDa; eIF3-p46; eIF3-p48; Eif3s6; Int6;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000022960

InnateDB Gene

IDBG-137965 (Eif3e)

Protein Structure

UniProt Annotation

Function

Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF- 3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF- 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. {ECO:0000250}.

Subcellular Localization

Cytoplasm {ECO:0000255HAMAP-Rule:MF_03004}. Nucleus, PML body {ECO:0000255HAMAP-Rule:MF_03004}.

Disease Associations

Note=Int-6 serves as a site for viral integration of mouse mammary tumor virus (MMTV) in mammary tumors.

Tissue Specificity

Ubiquitously expressed.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 13 experimentally validated interaction(s) in this database.
They are also associated with 89 interaction(s) predicted by orthology.

Experimentally validated
Total	13 [view]
Protein-Protein	13 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	89 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003743	translation initiation factor activity
GO:0005515	protein binding
GO:0044822	poly(A) RNA binding
GO:0047485	protein N-terminus binding

Biological Process

GO:0000184	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0001731	formation of translation preinitiation complex
GO:0006413	translational initiation
GO:0006446	regulation of translational initiation

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005852	eukaryotic translation initiation factor 3 complex
GO:0016020	membrane
GO:0016282	eukaryotic 43S preinitiation complex
GO:0016605	PML body
GO:0033290	eukaryotic 48S preinitiation complex
GO:0070062	extracellular vesicular exosome

Protein Structure and Domains

PDB ID

MGI:99257

InterPro

IPR000717 Proteasome component (PCI) domain
IPR016650 Eukaryotic translation initiation factor 3 subunit E
IPR019010 Eukaryotic translation initiation factor 3 subunit E, N-terminal

PFAM

PF01399
PF09440

PRINTS

PIRSF

PIRSF016255

SMART

SM00088

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt