InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Plk4

Summary

InnateDB Protein

IDBP-141447.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Plk4

Protein Name

polo-like kinase 4 (Drosophila)

Synonyms

1700028H20; AI385771; Sak; Stk18;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000026858

InnateDB Gene

IDBG-141445 (Plk4)

Protein Structure

UniProt Annotation

Function

Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Phosphorylates CDC25C and CHEK2. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. {ECO:0000269PubMed:17891141, ECO:0000269PubMed:24240477}.

Subcellular Localization

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Nucleus, nucleolus. Cleavage furrow. Note=Associates with centrioles throughout the cell cycle. According to PubMed:11301255, it localizes to the nucleolus during G2, to the centrosomes in G2/M, and to the cleavage furrow during cytokinesis. Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles.

Disease Associations

Tissue Specificity

expressed in tissues associated with mitotic and meiotic cell division. Highly expressed in testis. {ECO:0000269PubMed:8022793}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 12 experimentally validated interaction(s) in this database.
They are also associated with 13 interaction(s) predicted by orthology.

Experimentally validated
Total	12 [view]
Protein-Protein	12 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	13 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0042802	identical protein binding

Biological Process

GO:0006468	protein phosphorylation
GO:0007099	centriole replication
GO:0018108	peptidyl-tyrosine phosphorylation
GO:0046601	positive regulation of centriole replication
GO:0060707	trophoblast giant cell differentiation
GO:0098535	de novo centriole assembly

Cellular Component

GO:0005730	nucleolus
GO:0005813	centrosome
GO:0005814	centriole
GO:0032154	cleavage furrow
GO:0098536	deuterosome

Protein Structure and Domains

PDB ID

MGI:101783

InterPro

IPR000719 Protein kinase domain
IPR000959 POLO box duplicated domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00069
PF00659
PF07714

PRINTS

PR00109

PIRSF

SMART

SM00220
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt