InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Thoc5

Summary

InnateDB Protein

IDBP-143488.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Thoc5

Protein Name

THO complex 5

Synonyms

1700060C24Rik; A430085L24Rik; Fmip; PK1.3; Sgk2;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000045580

InnateDB Gene

IDBG-143486 (Thoc5)

Protein Structure

UniProt Annotation

Function

Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes.Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.

Subcellular Localization

Nucleus {ECO:0000269PubMed:15221008}. Nucleus speckle {ECO:0000250}. Cytoplasm {ECO:0000269PubMed:15221008}. Note=Shuttles between nucleus and cytoplasm.

Disease Associations

Tissue Specificity

Ubiquitously expressed, with highest levels in testis, liver and heart. {ECO:0000269PubMed:10597251, ECO:0000269PubMed:15451025}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 9 experimentally validated interaction(s) in this database.
They are also associated with 26 interaction(s) predicted by orthology.

Experimentally validated
Total	9 [view]
Protein-Protein	9 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	26 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003729	mRNA binding
GO:0005515	protein binding

Biological Process

GO:0000902	cell morphogenesis
GO:0001824	blastocyst development
GO:0006397	mRNA processing
GO:0006406	mRNA export from nucleus
GO:0008380	RNA splicing
GO:0010468	regulation of gene expression
GO:0010793	regulation of mRNA export from nucleus
GO:0017145	stem cell division
GO:0030224	monocyte differentiation
GO:0032786	positive regulation of DNA-templated transcription, elongation
GO:0045650	negative regulation of macrophage differentiation
GO:0046784	intronless viral mRNA export from host nucleus
GO:0060215	primitive hemopoiesis
GO:2000002	negative regulation of DNA damage checkpoint
GO:2000035	regulation of stem cell division

Cellular Component

GO:0000346	transcription export complex
GO:0000347	THO complex
GO:0000445	THO complex part of transcription export complex
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0016607	nuclear speck
GO:0045111	intermediate filament cytoskeleton