InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Trim28

Summary

InnateDB Protein

IDBP-143985.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Trim28

Protein Name

tripartite motif-containing 28

Synonyms

AA408787; KAP-1; KRIP-1; MommeD9; Tif1b; Tif1beta;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000005705

InnateDB Gene

IDBG-143983 (Trim28)

Protein Structure

UniProt Annotation

Function

Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:10330177, ECO:0000269PubMed:11154279, ECO:0000269PubMed:12154074}. Note=Associated with centromeric heterochromatin during cell differentiation through CBX1.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 85 experimentally validated interaction(s) in this database.
They are also associated with 139 interaction(s) predicted by orthology.

Experimentally validated
Total	85 [view]
Protein-Protein	85 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	139 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0003713	transcription coactivator activity
GO:0003714	transcription corepressor activity
GO:0004672	protein kinase activity
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016874	ligase activity
GO:0031625	ubiquitin protein ligase binding
GO:0035851	Krueppel-associated box domain binding
GO:0043565	sequence-specific DNA binding
GO:0044822	poly(A) RNA binding
GO:0070087	chromo shadow domain binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0001837	epithelial to mesenchymal transition
GO:0006281	DNA repair
GO:0006351	transcription, DNA-templated
GO:0006468	protein phosphorylation
GO:0016567	protein ubiquitination
GO:0016925	protein sumoylation
GO:0042993	positive regulation of transcription factor import into nucleus
GO:0045087	innate immune response
GO:0045739	positive regulation of DNA repair
GO:0045892	negative regulation of transcription, DNA-templated
GO:0045893	positive regulation of transcription, DNA-templated
GO:0046777	protein autophosphorylation
GO:0051259	protein oligomerization
GO:0060028	convergent extension involved in axis elongation
GO:0060669	embryonic placenta morphogenesis
GO:1902187	negative regulation of viral release from host cell

Cellular Component

GO:0000785	chromatin
GO:0005622	intracellular
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005719	nuclear euchromatin
GO:0005720	nuclear heterochromatin

Protein Structure and Domains

PDB ID

MGI:109274

InterPro

IPR000104 Antifreeze protein, type I
IPR000315 Zinc finger, B-box
IPR001487 Bromodomain
IPR001841 Zinc finger, RING-type
IPR001965 Zinc finger, PHD-type
IPR003649 B-box, C-terminal
IPR011011 Zinc finger, FYVE/PHD-type
IPR019787 Zinc finger, PHD-finger

PFAM

PF00643
PF00439
PF13639
PF14634
PF00628

PRINTS

PR00308
PR00503

PIRSF

SMART

SM00336
SM00297
SM00184
SM00249
SM00502

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt