InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Vrk2

Summary

InnateDB Protein

IDBP-156165.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Vrk2

Protein Name

vaccinia related kinase 2

Synonyms

2810003O05Rik; AI447698;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000077471

InnateDB Gene

IDBG-156163 (Vrk2)

Protein Structure

UniProt Annotation

Function

Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates histone H3. Phosphorylates 'Thr-18' of p53/TP53, and thereby increases its stability and activity. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. May also phosphorylate MAPK8IP1. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:14645249}. Endoplasmic reticulum membrane {ECO:0000305}; Single-pass type IV membrane protein {ECO:0000305}. Mitochondrion membrane {ECO:0000305}; Single-pass type IV membrane protein {ECO:0000305}.

Disease Associations

Tissue Specificity

Expressed in liver, kidney and muscle. Weakly expressed in thymus, bone marrow and spleen. {ECO:0000269PubMed:12782311}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 1 experimentally validated interaction(s) in this database.
They are also associated with 15 interaction(s) predicted by orthology.

Experimentally validated
Total	1 [view]
Protein-Protein	1 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	15 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups

Biological Process

GO:0006468	protein phosphorylation
GO:0034599	cellular response to oxidative stress
GO:0043408	regulation of MAPK cascade
GO:0046777	protein autophosphorylation
GO:2000659	regulation of interleukin-1-mediated signaling pathway

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005783	endoplasmic reticulum
GO:0005789	endoplasmic reticulum membrane
GO:0016020	membrane
GO:0016021	integral component of membrane
GO:0031966	mitochondrial membrane

Protein Structure and Domains

PDB ID

MGI:1917172

InterPro

IPR000719 Protein kinase domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain

PFAM

PF00069
PF07714

PRINTS

PR00109

PIRSF

SMART

SM00220

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt