InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Dna2

Summary

InnateDB Protein

IDBP-158347.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Dna2

Protein Name

DNA replication helicase 2 homolog (yeast)

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000090119

InnateDB Gene

IDBG-158343 (Dna2)

Protein Structure

UniProt Annotation

Function

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double- strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)- dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus {ECO:0000250}. Mitochondrion {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 4 experimentally validated interaction(s) in this database.
They are also associated with 2 interaction(s) predicted by orthology.

Experimentally validated
Total	4 [view]
Protein-Protein	4 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	2 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003678	DNA helicase activity
GO:0004518	nuclease activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016890	site-specific endodeoxyribonuclease activity, specific for altered base
GO:0017108	5'-flap endonuclease activity
GO:0043139	5'-3' DNA helicase activity
GO:0043142	single-stranded DNA-dependent ATPase activity
GO:0046872	metal ion binding
GO:0051539	4 iron, 4 sulfur cluster binding

Biological Process

GO:0000076	DNA replication checkpoint
GO:0000729	DNA double-strand break processing
GO:0000737	DNA catabolic process, endonucleolytic
GO:0006200	ATP catabolic process
GO:0006260	DNA replication
GO:0006264	mitochondrial DNA replication
GO:0006284	base-excision repair
GO:0032508	DNA duplex unwinding
GO:0033567	DNA replication, Okazaki fragment processing
GO:0043137	DNA replication, removal of RNA primer
GO:0043504	mitochondrial DNA repair
GO:0045740	positive regulation of DNA replication

Cellular Component

GO:0005634	nucleus
GO:0005760	gamma DNA polymerase complex
GO:0042645	mitochondrial nucleoid

Protein Structure and Domains

PDB ID

MGI:2443732

InterPro

IPR014808 DNA replication factor Dna2, N-terminal
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF08696

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q6ZQJ5

PhosphoSite

PhosphoSite-Q6ZQJ5

TrEMBL

UniProt Splice Variant

Entrez Gene

327762

UniGene

Mm.21492

RefSeq

MGI ID

MGI Symbol

Dna2

OMIM

CCDS

HPRD

IMGT

EMBL

AK028381 AK129051 BC025182 BC115716

GenPept

AAH25182 AAI15717 BAC25919 BAC97861