InnateDB Protein
|
IDBP-16065.6
|
Last Modified
|
2014-10-13 [Report errors or provide feedback]
|
Gene Symbol
|
HUS1
|
Protein Name
|
HUS1 checkpoint homolog (S. pombe)
|
Synonyms
|
hHUS1;
|
Species
|
Homo sapiens
|
Ensembl Protein
|
ENSP00000258774
|
InnateDB Gene
|
IDBG-16063 (HUS1)
|
Protein Structure
|
|
Function |
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. {ECO:0000269PubMed:21659603}.
|
Subcellular Localization |
Nucleus. Cytoplasm. Note=In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
|
Disease Associations |
|
Tissue Specificity |
Ubiquitous. {ECO:0000269PubMed:10777662}.
|
Comments |
|
Number of Interactions
|
This gene and/or its encoded proteins are associated with 38 experimentally validated interaction(s) in this database.
Experimentally validated |
Total |
38
[view]
|
Protein-Protein |
36
[view]
|
Protein-DNA |
2
[view]
|
Protein-RNA |
0
|
DNA-DNA |
0
|
RNA-RNA |
0
|
DNA-RNA |
0
|
|
|
|
Molecular Function |
|
Biological Process |
|
Cellular Component |
|
PDB ID |
|
InterPro |
IPR007150
Checkpoint protein Hus1/Mec3
IPR016580
Cell cycle checkpoint, Hus1
|
PFAM |
PF04005
|
PRINTS |
|
PIRSF |
PIRSF011312
|
SMART |
|
TIGRFAMs |
|
Modification |
|
SwissProt |
O60921
|
PhosphoSite |
PhosphoSite-O60921
|
TrEMBL |
C9JCK8
|
UniProt Splice Variant |
|
Entrez Gene |
3364
|
UniGene |
Hs.152983
|
RefSeq |
NP_004498
|
HUGO |
HGNC:5309
|
OMIM |
603760
|
CCDS |
CCDS34635
|
HPRD |
04787
|
IMGT |
|
EMBL |
AC019066
AC069282
AC104696
AF076844
AF110393
AF503165
AK290459
AK294117
BC007013
BT019481
BT019482
CH236958
CH471128
CR536552
Y16893
|
GenPept |
AAC95526
AAD25350
AAH07013
AAM18968
AAV38288
AAV38289
BAF83148
BAG57450
CAA76518
CAG38789
EAL23807
EAW61001
EAW61002
|
|
|