InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Sqstm1

Summary

InnateDB Protein

IDBP-168362.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Sqstm1

Protein Name

sequestosome 1

Synonyms

A170; OSF-6; Osi; p62; STAP; STONE14;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000015981

InnateDB Gene

IDBG-168358 (Sqstm1)

Protein Structure

UniProt Annotation

Function

Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin- 1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Adapter that mediates the interaction between TRAF6 and CYLD. {ECO:0000269PubMed:14960283, ECO:0000269PubMed:18382763}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:16286508}. Late endosome {ECO:0000269PubMed:16286508}. Nucleus {ECO:0000269PubMed:16286508}. Endoplasmic reticulum {ECO:0000269PubMed:16286508}. Lysosome {ECO:0000250}. Cytoplasmic vesicle, autophagosome {ECO:0000250}. Cytoplasm, P-body {ECO:0000250}. Note=Sarcomere. In cardiac muscles localizes to the sarcomeric band. May also localize to the hepatocellular carcinoma. Colocalizes with TRIM13 in the perinuclear endoplasmic reticulum (By similarity). Commonly found in inclusion bodies containing polyubiquitinated protein aggregates (By similarity). Co-localizes with TRIM5 in the cytoplasmic bodies (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Widely expressed. {ECO:0000269PubMed:10458914}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 98 experimentally validated interaction(s) in this database.
They are also associated with 172 interaction(s) predicted by orthology.

Experimentally validated
Total	98 [view]
Protein-Protein	98 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	172 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003712	transcription cofactor activity
GO:0005080	protein kinase C binding
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0042169	SH2 domain binding
GO:0042802	identical protein binding
GO:0042803	protein homodimerization activity
GO:0043130	ubiquitin binding
GO:0070530	K63-linked polyubiquitin binding

Biological Process

GO:0001934	positive regulation of protein phosphorylation
GO:0002376	immune system process
GO:0006914	autophagy
GO:0006915	apoptotic process
GO:0016236	macroautophagy
GO:0030154	cell differentiation
GO:0043122	regulation of I-kappaB kinase/NF-kappaB signaling
GO:0045087	innate immune response (InnateDB)
GO:0051291	protein heterooligomerization
GO:2001141	regulation of RNA biosynthetic process

Cellular Component

GO:0000407	pre-autophagosomal structure
GO:0000932	cytoplasmic mRNA processing body
GO:0005634	nucleus
GO:0005764	lysosome
GO:0005770	late endosome
GO:0005776	autophagic vacuole
GO:0005783	endoplasmic reticulum
GO:0016235	aggresome
GO:0031410	cytoplasmic vesicle

Protein Structure and Domains

PDB ID

MGI:107931

InterPro

IPR000270 Phox/Bem1p
IPR000433 Zinc finger, ZZ-type
IPR009060 UBA-like
IPR015940 Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote

PFAM

PF00564
PF00569

PRINTS

PIRSF

SMART

SM00666
SM00291
SM00165

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q64337