InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Htr2a

Summary

InnateDB Protein

IDBP-180293.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Htr2a

Protein Name

5-hydroxytryptamine (serotonin) receptor 2A

Synonyms

E030013E04; Htr-2; Htr2;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000047774

InnateDB Gene

IDBG-180291 (Htr2a)

Protein Structure

UniProt Annotation

Function

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5- dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca(2+) ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction. {ECO:0000269PubMed:16873667, ECO:0000269PubMed:18297054, ECO:0000269PubMed:21645528, ECO:0000269PubMed:23129762, ECO:0000269PubMed:23346101}.

Subcellular Localization

Cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Cell projection, axon {ECO:0000250}. Cytoplasmic vesicle {ECO:0000250}. Membrane, caveola {ECO:0000250}. Note=Localizes to the postsynaptic thickening of axo-dendritic synapses.

Disease Associations

Tissue Specificity

Detected in neurons in brain cortex. Detected in adult intestine, especially in mucosal epithelium, longitudinal and circular layers of muscularis externa and myenteric plexuses. Highly expressed in Paneth cells, and detected at lower levels in enterocytes (at protein level). Detected in neurons in the brain cortex. {ECO:0000269PubMed:11960784, ECO:0000269PubMed:18297054, ECO:0000269PubMed:23129762}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 4 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.

Experimentally validated
Total	4 [view]
Protein-Protein	3 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	4 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0001965	G-protein alpha-subunit binding
GO:0004930	G-protein coupled receptor activity
GO:0004993	serotonin receptor activity
GO:0005515	protein binding
GO:0008144	drug binding
GO:0032403	protein complex binding
GO:0051378	serotonin binding
GO:0071886	1-(4-iodo-2,5-dimethoxyphenyl)propan-2-amine binding

Biological Process

GO:0001659	temperature homeostasis
GO:0006874	cellular calcium ion homeostasis
GO:0007186	G-protein coupled receptor signaling pathway
GO:0007202	activation of phospholipase C activity
GO:0007204	positive regulation of cytosolic calcium ion concentration
GO:0007208	phospholipase C-activating serotonin receptor signaling pathway
GO:0007210	serotonin receptor signaling pathway
GO:0007568	aging
GO:0007613	memory
GO:0008219	cell death
GO:0008284	positive regulation of cell proliferation
GO:0010513	positive regulation of phosphatidylinositol biosynthetic process
GO:0014059	regulation of dopamine secretion
GO:0014065	phosphatidylinositol 3-kinase signaling
GO:0014824	artery smooth muscle contraction
GO:0014832	urinary bladder smooth muscle contraction
GO:0019233	sensory perception of pain
GO:0030431	sleep
GO:0033674	positive regulation of kinase activity
GO:0042493	response to drug
GO:0043267	negative regulation of potassium ion transport
GO:0043406	positive regulation of MAP kinase activity
GO:0044380	protein localization to cytoskeleton
GO:0045821	positive regulation of glycolytic process
GO:0045907	positive regulation of vasoconstriction
GO:0046883	regulation of hormone secretion
GO:0048148	behavioral response to cocaine
GO:0050731	positive regulation of peptidyl-tyrosine phosphorylation
GO:0050795	regulation of behavior
GO:0050965	detection of temperature stimulus involved in sensory perception of pain
GO:0050966	detection of mechanical stimulus involved in sensory perception of pain
GO:0051209	release of sequestered calcium ion into cytosol
GO:0051967	negative regulation of synaptic transmission, glutamatergic
GO:0070374	positive regulation of ERK1 and ERK2 cascade

Cellular Component

GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005886	plasma membrane
GO:0005887	integral component of plasma membrane
GO:0005901	caveola
GO:0016020	membrane
GO:0016021	integral component of membrane
GO:0016023	cytoplasmic membrane-bounded vesicle
GO:0030424	axon
GO:0030425	dendrite
GO:0043025	neuronal cell body
GO:0043198	dendritic shaft
GO:0070852	cell body fiber

Protein Structure and Domains

PDB ID

MGI:109521

InterPro

IPR000276 G protein-coupled receptor, rhodopsin-like
IPR000455 5-Hydroxytryptamine 2A receptor
IPR002231 5-hydroxytryptamine receptor family
IPR017452 GPCR, rhodopsin-like, 7TM
IPR019424 7TM GPCR, olfactory receptor/chemoreceptor Srsx
IPR019430 7TM GPCR, serpentine receptor class x (Srx)

PFAM

PF00001
PF10320
PF10328

PRINTS

PR00237
PR00516
PR01101

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P35363