InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: TGS1

Summary

InnateDB Protein

IDBP-22151.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

TGS1

Protein Name

trimethylguanosine synthase 1

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000260129

InnateDB Gene

IDBG-22149 (TGS1)

Protein Structure

UniProt Annotation

Function

Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation. {ECO:0000269PubMed:11517327, ECO:0000269PubMed:11912212, ECO:0000269PubMed:16687569, ECO:0000269PubMed:18775984}.

Subcellular Localization

Cytoplasm. Nucleus, Cajal body. Note=A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network.

Disease Associations

Tissue Specificity

Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta. {ECO:0000269PubMed:11517327}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 18 experimentally validated interaction(s) in this database.

Experimentally validated
Total	18 [view]
Protein-Protein	18 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0008168	methyltransferase activity
GO:0016740	transferase activity
GO:0071164	RNA trimethylguanosine synthase activity

Biological Process

GO:0000387	spliceosomal snRNP assembly
GO:0001510	RNA methylation
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0009452	7-methylguanosine RNA capping
GO:0010467	gene expression
GO:0016070	RNA metabolic process
GO:0022613	ribonucleoprotein complex biogenesis
GO:0034660	ncRNA metabolic process
GO:0036261	7-methylguanosine cap hypermethylation
GO:0044255	cellular lipid metabolic process
GO:0044281	small molecule metabolic process
GO:0071167	ribonucleoprotein complex import into nucleus

Cellular Component

GO:0005615	extracellular space
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0015030	Cajal body
GO:0030532	small nuclear ribonucleoprotein complex

Protein Structure and Domains

PDB ID

InterPro

IPR000241 Putative RNA methylase domain
IPR003402 tRNA transferase Trm5/Tyw2
IPR019012 RNA cap guanine-N2 methyltransferase
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase-like

PFAM

PF01170
PF02475
PF09445

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q96RS0

PhosphoSite