InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RASSF1

Summary

InnateDB Protein

IDBP-240177.7

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RASSF1

Protein Name

Ras association (RalGDS/AF-6) domain family member 1

Synonyms

123F2; NORE2A; RASSF1A; RDA32; REH3P21;

Species

Homo sapiens

Ensembl Protein

ENSP00000378549

InnateDB Gene

IDBG-36632 (RASSF1)

Protein Structure

UniProt Annotation

Function

Potential tumor suppressor. Required for death receptor- dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. {ECO:0000269PubMed:10888881, ECO:0000269PubMed:11333291, ECO:0000269PubMed:12024041, ECO:0000269PubMed:14743218, ECO:0000269PubMed:15109305, ECO:0000269PubMed:15949439, ECO:0000269PubMed:16510573, ECO:0000269PubMed:18566590, ECO:0000269PubMed:21199877}.

Subcellular Localization

Isoform A: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Nucleus. Note=Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.Isoform C: Nucleus. Note=Predominantly nuclear.

Disease Associations

Tissue Specificity

Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells. {ECO:0000269PubMed:10888881, ECO:0000269PubMed:11333291}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 79 experimentally validated interaction(s) in this database.
They are also associated with 3 interaction(s) predicted by orthology.

Experimentally validated
Total	79 [view]
Protein-Protein	76 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	3 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0042802	identical protein binding
GO:0047485	protein N-terminus binding

Biological Process

GO:0006974	cellular response to DNA damage stimulus
GO:0007050	cell cycle arrest
GO:0007265	Ras protein signal transduction
GO:0031398	positive regulation of protein ubiquitination
GO:0035556	intracellular signal transduction
GO:0050821	protein stabilization
GO:0071157	negative regulation of cell cycle arrest

Cellular Component

GO:0000922	spindle pole
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005815	microtubule organizing center
GO:0005874	microtubule
GO:0005886	plasma membrane
GO:0015630	microtubule cytoskeleton