InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: USP44

Summary

InnateDB Protein

IDBP-244330.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

USP44

Protein Name

ubiquitin specific peptidase 44

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000376806

InnateDB Gene

IDBG-51735 (USP44)

Protein Structure

UniProt Annotation

Function

Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination. {ECO:0000269PubMed:17443180, ECO:0000269PubMed:22681888}.

Subcellular Localization

Nucleus {ECO:0000269PubMed:20402667}. Note=Peaks in interphase, with relatively low levels maintained throughout mitosis. {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed in testis. Expressed at high levels in T-cell acute lymphoblastic leukemia. {ECO:0000269PubMed:14715245, ECO:0000269PubMed:21853124}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 12 experimentally validated interaction(s) in this database.

Experimentally validated
Total	12 [view]
Protein-Protein	12 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004221	ubiquitin thiolesterase activity
GO:0004843	ubiquitin-specific protease activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0036459	ubiquitinyl hydrolase activity

Biological Process

GO:0006511	ubiquitin-dependent protein catabolic process
GO:0007067	mitotic nuclear division
GO:0016579	protein deubiquitination
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0051322	anaphase
GO:0060564	negative regulation of mitotic anaphase-promoting complex activity
GO:0090231	regulation of spindle checkpoint
GO:0090266	regulation of mitotic cell cycle spindle assembly checkpoint

Cellular Component

GO:0005634

nucleus

Protein Structure and Domains

PDB ID

InterPro

IPR001394 Peptidase C19, ubiquitin carboxyl-terminal hydrolase
IPR001607 Zinc finger, UBP-type
IPR028889 Ubiquitin carboxyl-terminal hydrolase-like domain

PFAM

PF00443
PF02148

PRINTS

PIRSF

SMART

SM00290

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9H0E7