InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Diap1

Summary

InnateDB Protein

IDBP-249190.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Diap1

Protein Name

diaphanous homolog 1 (Drosophila)

Synonyms

D18Wsu154e; Dia1; Diaph1; Drf1; mKIAA4062; p140mDia;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000111297

InnateDB Gene

IDBG-140703 (Diap1)

Protein Structure

UniProt Annotation

Function

Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration. Has neurite outgrowth promoting activity. The MEMO1- RHOA-DIAPH1 signaling pathway plays an important role in ERBB2- dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (By similarity). {ECO:0000250}.

Subcellular Localization

Cell membrane {ECO:0000269PubMed:9214622}. Cell projection, ruffle membrane {ECO:0000269PubMed:9214622}. Cytoplasm, cytoskeleton {ECO:0000269PubMed:9214622}. Note=Membrane ruffles, especially at the tip of ruffles, of motile cells.

Disease Associations

Tissue Specificity

Ubiquitous.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 33 experimentally validated interaction(s) in this database.
They are also associated with 23 interaction(s) predicted by orthology.

Experimentally validated
Total	33 [view]
Protein-Protein	32 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	23 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003779	actin binding
GO:0005488	binding
GO:0005515	protein binding
GO:0005522	profilin binding
GO:0017048	Rho GTPase binding
GO:0042802	identical protein binding
GO:0044325	ion channel binding

Biological Process

GO:0007010	cytoskeleton organization
GO:0007605	sensory perception of sound
GO:0008360	regulation of cell shape
GO:0016043	cellular component organization
GO:0030036	actin cytoskeleton organization
GO:0030041	actin filament polymerization
GO:0030335	positive regulation of cell migration
GO:0031175	neuron projection development
GO:0032886	regulation of microtubule-based process
GO:0035372	protein localization to microtubule
GO:0051279	regulation of release of sequestered calcium ion into cytosol
GO:0071420	cellular response to histamine

Cellular Component

GO:0005737	cytoplasm
GO:0032587	ruffle membrane
GO:0043005	neuron projection
GO:0072686	mitotic spindle

Protein Structure and Domains

PDB ID

MGI:1194490

InterPro

IPR009408 Formin Homology 1
IPR010465 DRF autoregulatory
IPR010472 Formin, FH3 domain
IPR010473 Formin, GTPase-binding domain
IPR015425 Formin, FH2 domain
IPR016024 Armadillo-type fold

PFAM

PF06346
PF06345
PF06367
PF06371
PF02181

PRINTS

PIRSF

SMART

SM00498

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

O08808