InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Hck

Summary

InnateDB Protein

IDBP-261615.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Hck

Protein Name

hemopoietic cell kinase

Synonyms

AI849071; Bmk;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000105423

InnateDB Gene

IDBG-209984 (Hck)

Protein Structure

UniProt Annotation

Function

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS (By similarity). {ECO:0000250}.

Subcellular Localization

Cytoplasmic vesicle, secretory vesicle {ECO:0000250}. Cytoplasm, cytosol {ECO:0000250}.Isoform 1: Membrane; Lipid-anchor. Membrane, caveola {ECO:0000250}. Lysosome {ECO:0000250}. Cell projection, podosome membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}. Cytoplasm, cytosol {ECO:0000250}. Note=Associated with specialized secretory lysosomes called azurophil granules. A fraction of this isoform is found in the cytoplasm, some of this fraction is myristoylated (By similarity). {ECO:0000250}.Isoform 2: Cell membrane; Lipid-anchor. Membrane, caveola {ECO:0000250}; Lipid-anchor {ECO:0000250}. Cell junction, focal adhesion {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Golgi apparatus {ECO:0000250}. Cytoplasmic vesicle {ECO:0000250}. Lysosome {ECO:0000250}. Nucleus {ECO:0000250}. Note=20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions (By similarity). {ECO:0000250}.

Disease Associations

Tissue Specificity

Expressed predominantly in cells of the myeloid and B-lymphoid lineages.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 17 experimentally validated interaction(s) in this database.
They are also associated with 66 interaction(s) predicted by orthology.

Experimentally validated
Total	17 [view]
Protein-Protein	17 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	66 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0004715	non-membrane spanning protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups

Biological Process

GO:0006468	protein phosphorylation
GO:0006887	exocytosis
GO:0006909	phagocytosis
GO:0006954	inflammatory response
GO:0008284	positive regulation of cell proliferation
GO:0008360	regulation of cell shape
GO:0018108	peptidyl-tyrosine phosphorylation
GO:0045087	innate immune response
GO:0046777	protein autophosphorylation
GO:0050764	regulation of phagocytosis
GO:0050830	defense response to Gram-positive bacterium
GO:0071801	regulation of podosome assembly
GO:2000251	positive regulation of actin cytoskeleton reorganization

Cellular Component

GO:0005634	nucleus
GO:0005764	lysosome
GO:0005794	Golgi apparatus
GO:0005829	cytosol
GO:0005884	actin filament
GO:0005901	caveola
GO:0005925	focal adhesion
GO:0030133	transport vesicle
GO:0031234	extrinsic component of cytoplasmic side of plasma membrane
GO:0042995	cell projection

Protein Structure and Domains

PDB ID

MGI:96052

InterPro

IPR000719 Protein kinase domain
IPR000980 SH2 domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR001452 SH3 domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR011511 Variant SH3 domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM