InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Pou2f2

Summary

InnateDB Protein

IDBP-264581.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Pou2f2

Protein Name

POU domain, class 2, transcription factor 2

Synonyms

Oct-2; Oct2a; Oct2b; Otf-2; Otf2;

Species

Mus musculus

Ensembl Protein

ENSMUSP00000104055

InnateDB Gene

IDBG-156347 (Pou2f2)

Protein Structure

UniProt Annotation

Function

Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform OCT2.5 activates the U2 small nuclear RNA (snRNA) promoter. Isoforms OCT2.1, OCT2.2 and OCT2.3 activate octamer- containing promoters. Isoforms OCT2.4 and OCT2.5 repress some promoters and activate others. Isoform OCT2.7 is unable to bind to the octamer motif, but can still activate the beta-casein gene promoter at low levels. {ECO:0000269PubMed:1281152, ECO:0000269PubMed:17285328, ECO:0000269PubMed:2011512, ECO:0000269PubMed:7935477}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:17285328}. Nucleus {ECO:0000255PROSITE-ProRule:PRU00108, ECO:0000255PROSITE-ProRule:PRU00530, ECO:0000269PubMed:17285328}. Note=In alveolus epithelial cells of mammary glands, present in the nucleus and cytoplasm. In HC11 mammary epithelial cells, present in the nucleus and preinuclear regions.

Disease Associations

Tissue Specificity

Highest in B-cells, but also present in brain (neuronal and glial cells), intestine, kidney, and testes. Isoform OCT2.1 is expressed at higher levels in B-cells than in neuronal cells. Isoform OCT2.2 is expressed in neuronal cell lines and brain, but not dorsal root ganglia. Isoform OCT2.3 is expressed at lower levels in neuronal cells than in B-cells. Isoform OCT2.4 is expressed in neuronal cell lines, and at lower levels in neuroblastoma and dorsal root ganglia. Isoform OCT2.5 is widely expressed in the developing nervous system but expression is confined to very specific regions in the adult brain, it is expressed at a lower level in B-cells. Isoform OCT2.6 is either absent in, or expressed at very low levels in neuronal cells and brain. Isoform OCT2.7 is expressed in all tissues tested: mammary gland, liver, spleen, lung, kidney intestine, uterus and ovary of a virgin mouse. Levels of isoform OCT2.7 are highest in spleen and lung. In mammary gland, expression is localized to the alveolus epithelial cells. {ECO:0000269PubMed:1281152, ECO:0000269PubMed:17285328}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 3 experimentally validated interaction(s) in this database.
They are also associated with 15 interaction(s) predicted by orthology.

Experimentally validated
Total	3 [view]
Protein-Protein	0
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	15 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003700	sequence-specific DNA binding transcription factor activity
GO:0019904	protein domain specific binding
GO:0043565	sequence-specific DNA binding

Biological Process

GO:0002335	mature B cell differentiation
GO:0002380	immunoglobulin secretion involved in immune response
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0045893	positive regulation of transcription, DNA-templated
GO:0048305	immunoglobulin secretion
GO:0048469	cell maturation

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm

Protein Structure and Domains

PDB ID

MGI:101897

InterPro

IPR000327 POU-specific
IPR000972 Octamer-binding transcription factor
IPR001356 Homeobox domain
IPR009057 Homeodomain-like
IPR010982 Lambda repressor-like, DNA-binding domain
IPR013847 POU domain

PFAM

PF00157
PF00046
PF13413

PRINTS

PR00029
PR00028

PIRSF

SMART

SM00352
SM00389

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt