InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: CARM1

Summary

InnateDB Protein

IDBP-28474.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

CARM1

Protein Name

coactivator-associated arginine methyltransferase 1

Synonyms

PRMT4;

Species

Homo sapiens

Ensembl Protein

ENSP00000325690

InnateDB Gene

IDBG-28472 (CARM1)

Protein Structure

UniProt Annotation

Function

Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg- 2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs. {ECO:0000269PubMed:16497732, ECO:0000269PubMed:19405910}.

Subcellular Localization

Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.

Disease Associations

Tissue Specificity

Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. {ECO:0000269PubMed:15221992}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 106 experimentally validated interaction(s) in this database.
They are also associated with 9 interaction(s) predicted by orthology.

Experimentally validated
Total	106 [view]
Protein-Protein	102 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	2 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	9 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003713	transcription coactivator activity
GO:0005515	protein binding
GO:0008013	beta-catenin binding
GO:0008168	methyltransferase activity
GO:0008276	protein methyltransferase activity
GO:0008469	histone-arginine N-methyltransferase activity
GO:0016274	protein-arginine N-methyltransferase activity
GO:0030374	ligand-dependent nuclear receptor transcription coactivator activity
GO:0035242	protein-arginine omega-N asymmetric methyltransferase activity
GO:0035642	histone methyltransferase activity (H3-R17 specific)
GO:0042054	histone methyltransferase activity
GO:0042803	protein homodimerization activity
GO:0044212	transcription regulatory region DNA binding
GO:0070577	lysine-acetylated histone binding

Biological Process

GO:0003420	regulation of growth plate cartilage chondrocyte proliferation
GO:0006351	transcription, DNA-templated
GO:0006355	regulation of transcription, DNA-templated
GO:0006479	protein methylation
GO:0008152	metabolic process
GO:0008284	positive regulation of cell proliferation
GO:0009405	pathogenesis
GO:0016032	viral process
GO:0016571	histone methylation
GO:0019919	peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
GO:0030518	intracellular steroid hormone receptor signaling pathway
GO:0030520	intracellular estrogen receptor signaling pathway
GO:0032091	negative regulation of protein binding
GO:0033146	regulation of intracellular estrogen receptor signaling pathway
GO:0034969	histone arginine methylation
GO:0034970	histone H3-R2 methylation
GO:0034971	histone H3-R17 methylation
GO:0035246	peptidyl-arginine N-methylation
GO:0044255	cellular lipid metabolic process
GO:0044281	small molecule metabolic process
GO:0045600	positive regulation of fat cell differentiation
GO:0051591	response to cAMP
GO:0060350	endochondral bone morphogenesis

Cellular Component

GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005829	cytosol

Protein Structure and Domains

PDB ID

InterPro

IPR007848 Methyltransferase small domain
IPR010456 Ribosomal L11 methyltransferase, PrmA
IPR013216 Methyltransferase type 11
IPR020989 Histone-arginine methyltransferase CARM1, N-terminal
IPR025799 Protein arginine N-methyltransferase
IPR027555 tRNA (mo5U34)-methyltransferase-like
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase-like

PFAM

PF05175
PF06325
PF08241
PF11531
PF05185
PF08003

PRINTS

PIRSF

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q86X55

PhosphoSite