Homo sapiens Protein: CARM1
InnateDB Protein IDBP-28474.5
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol CARM1
Protein Name coactivator-associated arginine methyltransferase 1
Synonyms PRMT4;
Species Homo sapiens
Ensembl Protein ENSP00000325690
InnateDB Gene IDBG-28472 (CARM1)
Protein Structure
UniProt Annotation
Function Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg- 2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA- stabilizing properties and the half-life of their target mRNAs. {ECO:0000269PubMed:16497732, ECO:0000269PubMed:19405910}.
Subcellular Localization Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
Disease Associations
Tissue Specificity Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. {ECO:0000269PubMed:15221992}.
Number of Interactions This gene and/or its encoded proteins are associated with 106 experimentally validated interaction(s) in this database.
They are also associated with 9 interaction(s) predicted by orthology.
Experimentally validated
Total 106 [view]
Protein-Protein 102 [view]
Protein-DNA 2 [view]
Protein-RNA 0
DNA-DNA 2 [view]
Predicted by orthology
Total 9 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003713 transcription coactivator activity
GO:0005515 protein binding
GO:0008013 beta-catenin binding
GO:0008168 methyltransferase activity
GO:0008276 protein methyltransferase activity
GO:0008469 histone-arginine N-methyltransferase activity
GO:0016274 protein-arginine N-methyltransferase activity
GO:0030374 ligand-dependent nuclear receptor transcription coactivator activity
GO:0035242 protein-arginine omega-N asymmetric methyltransferase activity
GO:0035642 histone methyltransferase activity (H3-R17 specific)
GO:0042054 histone methyltransferase activity
GO:0042803 protein homodimerization activity
GO:0044212 transcription regulatory region DNA binding
GO:0070577 lysine-acetylated histone binding
Biological Process
GO:0003420 regulation of growth plate cartilage chondrocyte proliferation
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006479 protein methylation
GO:0008152 metabolic process
GO:0008284 positive regulation of cell proliferation
GO:0009405 pathogenesis
GO:0016032 viral process
GO:0016571 histone methylation
GO:0019919 peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
GO:0030518 intracellular steroid hormone receptor signaling pathway
GO:0030520 intracellular estrogen receptor signaling pathway
GO:0032091 negative regulation of protein binding
GO:0033146 regulation of intracellular estrogen receptor signaling pathway
GO:0034969 histone arginine methylation
GO:0034970 histone H3-R2 methylation
GO:0034971 histone H3-R17 methylation
GO:0035246 peptidyl-arginine N-methylation
GO:0044255 cellular lipid metabolic process
GO:0044281 small molecule metabolic process
GO:0045600 positive regulation of fat cell differentiation
GO:0051591 response to cAMP
GO:0060350 endochondral bone morphogenesis
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
Protein Structure and Domains
InterPro IPR007848 Methyltransferase small domain
IPR010456 Ribosomal L11 methyltransferase, PrmA
IPR013216 Methyltransferase type 11
IPR020989 Histone-arginine methyltransferase CARM1, N-terminal
IPR025799 Protein arginine N-methyltransferase
IPR027555 tRNA (mo5U34)-methyltransferase-like
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase-like
PFAM PF05175
Post-translational Modifications
SwissProt Q86X55
PhosphoSite PhosphoSite-Q86X55
UniProt Splice Variant
Entrez Gene 10498
UniGene Hs.734431
RefSeq NP_954592
OMIM 603934
HPRD 09158
EMBL AC007565 AC011442 AL833242 BC046240 BC172490 CH471106
GenPept AAH46240 EAW84156