Version 5.4
Homo sapiens Protein: DGCR8
Summary
InnateDB Protein IDBP-292551.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol DGCR8
Protein Name DiGeorge syndrome critical region gene 8
Synonyms
Species Homo sapiens
Ensembl Protein ENSP00000384726
InnateDB Gene IDBG-1583 (DGCR8)
Protein Structure
UniProt Annotation
Function Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri- miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri- miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Involved in the silencing of embryonic stem cells self- renewal. {ECO:0000269PubMed:15531877, ECO:0000269PubMed:15574589, ECO:0000269PubMed:15589161, ECO:0000269PubMed:16751099, ECO:0000269PubMed:16906129, ECO:0000269PubMed:16963499, ECO:0000269PubMed:17159994}.
Subcellular Localization Nucleus. Nucleus, nucleolus. Note=Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.
Disease Associations
Tissue Specificity Ubiquitously expressed. {ECO:0000269PubMed:12705904}.
Comments
Interactions
Number of Interactions This gene and/or its encoded proteins are associated with 42 experimentally validated interaction(s) in this database.
They are also associated with 1 interaction(s) predicted by orthology.
Experimentally validated
Total 42 [view]
Protein-Protein 42 [view]
Protein-DNA 0
Protein-RNA 0
DNA-DNA 0
RNA-RNA 0
DNA-RNA 0
Predicted by orthology
Total 1 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003725 double-stranded RNA binding
GO:0005515 protein binding
GO:0046872 metal ion binding
Biological Process
GO:0010467 gene expression
GO:0031053 primary miRNA processing
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0015630 microtubule cytoskeleton
Protein Structure and Domains
PDB ID
InterPro IPR001202 WW domain
IPR014720 Double-stranded RNA-binding domain
PFAM PF00397
PF00035
PRINTS
PIRSF
SMART SM00456
SM00358
TIGRFAMs
Post-translational Modifications
Modification
Cross-References
SwissProt Q8WYQ5
PhosphoSite PhosphoSite-Q8WYQ5
TrEMBL C9JSD5
UniProt Splice Variant
Entrez Gene 54487
UniGene Hs.732256
RefSeq
HUGO HGNC:2847
OMIM 609030
CCDS CCDS54501
HPRD 09913
IMGT
EMBL AB050770 AC006547 AF165527 AK025539 AK025780 AK313357 AY189282 BC009323 BC009984 BC078147 BX649187 CR456356
GenPept AAF82263 AAH09323 AAH09984 AAH78147 AAO86726 BAB15165 BAB15238 BAB83032 BAG36158 CAE46205 CAG30242

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca