InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: HERC5

Summary

InnateDB Protein

IDBP-29778.5

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

HERC5

Protein Name

hect domain and RLD 5

Synonyms

Species

Homo sapiens

Ensembl Protein

ENSP00000264350

InnateDB Gene

IDBG-29776 (HERC5)

Protein Structure

UniProt Annotation

Function

Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant- negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15. {ECO:0000269PubMed:16407192, ECO:0000269PubMed:16815975, ECO:0000269PubMed:16884686, ECO:0000269PubMed:20133869, ECO:0000269PubMed:20308324, ECO:0000269PubMed:20385878, ECO:0000269PubMed:20542004}.

Subcellular Localization

Cytoplasm, perinuclear region {ECO:0000269PubMed:20542004}. Note=Associated with the polyribosomes, probably via the 60S subunit.

Disease Associations

Tissue Specificity

Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels. {ECO:0000269PubMed:10581175, ECO:0000269PubMed:15331633}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 24 experimentally validated interaction(s) in this database.

Experimentally validated
Total	24 [view]
Protein-Protein	23 [view]
Protein-DNA	1 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0016874	ligase activity
GO:0042296	ISG15 ligase activity
GO:0044822	poly(A) RNA binding

Biological Process

GO:0000079	regulation of cyclin-dependent protein serine/threonine kinase activity
GO:0016567	protein ubiquitination
GO:0019221	cytokine-mediated signaling pathway
GO:0032020	ISG15-protein conjugation
GO:0032480	negative regulation of type I interferon production
GO:0042787	protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0045087	innate immune response (InnateDB)
GO:0050688	regulation of defense response to virus
GO:0051607	defense response to virus

Cellular Component

GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0048471	perinuclear region of cytoplasm

Protein Structure and Domains

PDB ID

InterPro

IPR000408 Regulator of chromosome condensation, RCC1
IPR000569 HECT
IPR009091 Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II
IPR016084 Haem oxygenase-like, multi-helical

PFAM

PF00415
PF00632

PRINTS

PR00633

PIRSF

SMART

SM00119

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt