Homo sapiens Protein: DDX1
InnateDB Protein IDBP-30763.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol DDX1
Protein Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Synonyms DBP-RB; UKVH5d;
Species Homo sapiens
Ensembl Protein ENSP00000370745
InnateDB Gene IDBG-30761 (DDX1)
Protein Structure
UniProt Annotation
Function Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF- kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (PubMed:24870230). Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs. {ECO:0000269PubMed:12183465, ECO:0000269PubMed:15567440, ECO:0000269PubMed:18335541, ECO:0000269PubMed:18710941, ECO:0000269PubMed:20573827, ECO:0000269PubMed:24870230}.
Subcellular Localization Nucleus. Cytoplasm. Cytoplasmic granule. Note=Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR- induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM- dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low (PubMed:24608264). {ECO:0000269PubMed:24608264}.
Disease Associations
Tissue Specificity Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord. {ECO:0000269PubMed:7689221}.
Number of Interactions This gene and/or its encoded proteins are associated with 130 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.
Experimentally validated
Total 130 [view]
Protein-Protein 128 [view]
Protein-DNA 2 [view]
Protein-RNA 0
Predicted by orthology
Total 6 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003712 transcription cofactor activity
GO:0003724 RNA helicase activity
GO:0003725 double-stranded RNA binding
GO:0004518 nuclease activity
GO:0004527 exonuclease activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008026 ATP-dependent helicase activity
GO:0008143 poly(A) binding
GO:0033677 DNA/RNA helicase activity
GO:0044822 poly(A) RNA binding
Biological Process
GO:0000245 spliceosomal complex assembly
GO:0006200 ATP catabolic process
GO:0006302 double-strand break repair
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006446 regulation of translational initiation
GO:0007275 multicellular organismal development
GO:0009615 response to virus
GO:0032508 DNA duplex unwinding
GO:0043330 response to exogenous dsRNA
GO:0045087 innate immune response (InnateDB)
GO:0090305 nucleic acid phosphodiester bond hydrolysis
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0010494 cytoplasmic stress granule
GO:0016020 membrane
GO:0030529 ribonucleoprotein complex
GO:0071920 cleavage body
GO:0072669 tRNA-splicing ligase complex
Protein Structure and Domains
InterPro IPR001650 Helicase, C-terminal
IPR001870 B30.2/SPRY domain
IPR003877 SPRY domain
IPR008985 Concanavalin A-like lectin/glucanases superfamily
IPR011545 DEAD/DEAH box helicase domain
IPR014001 Helicase, superfamily 1/2, ATP-binding domain
IPR014014 RNA helicase, DEAD-box type, Q motif
IPR027417 P-loop containing nucleoside triphosphate hydrolase
PFAM PF00271
Post-translational Modifications
SwissProt Q92499
PhosphoSite PhosphoSite-Q92499
UniProt Splice Variant
Entrez Gene 1653
UniGene Hs.440599
OMIM 601257
HPRD 03158
EMBL AB593031 AB593032 AB593034 AK297432 AK313933 BC012132 BC053673 CH471053 EF418570 EF418571 X70649
GenPept AAH12132 AAH53673 ABN70940 ABN70941 BAG36652 BAG59860 BAJ83985 BAJ83986 BAJ83988 CAA49992 EAX00897