Homo sapiens Protein: DDX1
InnateDB Protein IDBP-30765.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol DDX1
Protein Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Synonyms DBP-RB; UKVH5d;
Species Homo sapiens
Ensembl Protein ENSP00000233084
InnateDB Gene IDBG-30761 (DDX1)
Protein Structure
UniProt Annotation
Function Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF- kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (PubMed:24870230). Required for HIV-1 Rev function as well as for HIV-1 replication. Binds to the RRE sequence of HIV-1 mRNAs. {ECO:0000269PubMed:12183465, ECO:0000269PubMed:15567440, ECO:0000269PubMed:18335541, ECO:0000269PubMed:18710941, ECO:0000269PubMed:20573827, ECO:0000269PubMed:24870230}.
Subcellular Localization Nucleus. Cytoplasm. Cytoplasmic granule. Note=Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR- induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM- dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells. Required for proper localization of HIV-1 Rev. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low (PubMed:24608264). {ECO:0000269PubMed:24608264}.
Disease Associations
Tissue Specificity Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord. {ECO:0000269PubMed:7689221}.
Number of Interactions This gene and/or its encoded proteins are associated with 130 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.
Experimentally validated
Total 130 [view]
Protein-Protein 128 [view]
Protein-DNA 2 [view]
Protein-RNA 0
Predicted by orthology
Total 6 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003712 transcription cofactor activity
GO:0003724 RNA helicase activity
GO:0004518 nuclease activity
GO:0004527 exonuclease activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008026 ATP-dependent helicase activity
GO:0008143 poly(A) binding
GO:0033677 DNA/RNA helicase activity
GO:0044822 poly(A) RNA binding
Biological Process
GO:0000245 spliceosomal complex assembly
GO:0006200 ATP catabolic process
GO:0006302 double-strand break repair
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006446 regulation of translational initiation
GO:0007275 multicellular organismal development
GO:0032508 DNA duplex unwinding
GO:0045087 innate immune response (InnateDB)
GO:0090305 nucleic acid phosphodiester bond hydrolysis
Cellular Component
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0010494 cytoplasmic stress granule
GO:0016020 membrane
GO:0030529 ribonucleoprotein complex
GO:0071920 cleavage body
GO:0072669 tRNA-splicing ligase complex
Protein Structure and Domains
InterPro IPR001650 Helicase, C-terminal
IPR001870 B30.2/SPRY domain
IPR003877 SPRY domain
IPR008985 Concanavalin A-like lectin/glucanases superfamily
IPR011545 DEAD/DEAH box helicase domain
IPR014001 Helicase, superfamily 1/2, ATP-binding domain
IPR014014 RNA helicase, DEAD-box type, Q motif
IPR027417 P-loop containing nucleoside triphosphate hydrolase
PFAM PF00271
Post-translational Modifications
SwissProt Q92499
PhosphoSite PhosphoSite-Q92499
UniProt Splice Variant
Entrez Gene 1653
UniGene Hs.440599
RefSeq NP_004930
OMIM 601257
HPRD 03158
EMBL AB593031 AB593032 AB593034 AK297432 AK313933 BC012132 BC053673 CH471053 EF418570 EF418571 X70649
GenPept AAH12132 AAH53673 ABN70940 ABN70941 BAG36652 BAG59860 BAJ83985 BAJ83986 BAJ83988 CAA49992 EAX00897