InnateDB Protein
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IDBP-32115.6
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Last Modified
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2014-10-13 [Report errors or provide feedback]
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Gene Symbol
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UBE2D3
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Protein Name
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ubiquitin-conjugating enzyme E2D 3
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Synonyms
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E2(17)KB3; UBC4/5; UBCH5C;
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Species
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Homo sapiens
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Ensembl Protein
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ENSP00000337208
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InnateDB Gene
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IDBG-32113 (UBE2D3)
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Protein Structure
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Function |
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA- linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML- NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction (By similarity). {ECO:0000250}.
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Subcellular Localization |
Cell membrane {ECO:0000269PubMed:18508924}; Peripheral membrane protein {ECO:0000269PubMed:18508924}. Endosome membrane {ECO:0000269PubMed:18508924}; Peripheral membrane protein {ECO:0000269PubMed:18508924}.
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Disease Associations |
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Tissue Specificity |
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Comments |
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Number of Interactions
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This gene and/or its encoded proteins are associated with 248 experimentally validated interaction(s) in this database.
Experimentally validated |
Total |
248
[view]
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Protein-Protein |
244
[view]
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Protein-DNA |
4
[view]
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Protein-RNA |
0
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DNA-DNA |
0
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RNA-RNA |
0
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DNA-RNA |
0
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Molecular Function |
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Biological Process |
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Cellular Component |
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PDB ID |
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InterPro |
IPR000608
Ubiquitin-conjugating enzyme, E2
IPR016135
Ubiquitin-conjugating enzyme/RWD-like
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PFAM |
PF00179
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PRINTS |
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PIRSF |
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SMART |
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TIGRFAMs |
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Modification |
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SwissProt |
P61077
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PhosphoSite |
PhosphoSite-P61077
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TrEMBL |
D6RIZ3
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UniProt Splice Variant |
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Entrez Gene |
7323
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UniGene |
Hs.732195
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RefSeq |
NP_871619
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HUGO |
HGNC:12476
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OMIM |
602963
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CCDS |
CCDS3660
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HPRD |
04269
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IMGT |
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EMBL |
AC010378
AC018797
AF213884
AK095822
BC003395
BC037894
BC066917
CH471057
CR456916
DB045280
U39318
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GenPept |
AAA91461
AAF35234
AAH03395
AAH37894
AAH66917
BAC04632
CAG33197
EAX06138
EAX06139
EAX06141
EAX06142
EAX06143
EAX06144
EAX06145
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